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不同预处理方式对玉米秸秆酶解液成分的影响
引用本文:田双起,王振宇,左丽丽,樊梓鸾. 不同预处理方式对玉米秸秆酶解液成分的影响[J]. 中国酿造, 2012, 31(6): 140-143
作者姓名:田双起  王振宇  左丽丽  樊梓鸾
作者单位:1. 哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨,150090
2. 哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090;东北林业大学林学院,黑龙江哈尔滨150040
摘    要:玉米秸秆中的单糖或者寡糖的释放由于芳香族聚合物木质素阻遏纤维素酶进行糖解聚作用而效率地下.然而预处理作用可以有效地移除木质素或者打破木质素的结构,从而可以增强还原糖的释放.其过程主要包括2个生物转化程序:玉米秸秆中纤维素的水解产生还原糖,然后进行生物乙醇发酵生产过程.在该研究中,通过HPLC方法监测不同预处理方法处理玉米秸秆酶水解液中的成分,从而得到最佳的预处理方法.结果表明,磁力搅拌辅助CO2激光协同通气预处理方法的糖化效果高于其他预处理方法.

关 键 词:玉米秸秆  预处理  酶解液  HPLC

Effects of different pretreatment methods on chemical composition of corn stover hydrolysate
TIAN Shuangqi , WANG Zhenyu , ZUO Lili , FAN Ziluan. Effects of different pretreatment methods on chemical composition of corn stover hydrolysate[J]. China Brewing, 2012, 31(6): 140-143
Authors:TIAN Shuangqi    WANG Zhenyu    ZUO Lili    FAN Ziluan
Affiliation:1.School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China, 2.School of Forestry, Northeast Forestry University, Harbin 150040, China)
Abstract:Release of monosaccharide or oligosaccharide from corn stover biomass is inefficient because lignin, an aromatic polymer, blocks the ac-cess of cellnlase to sugar polymers. However, pretreatments can remove lignin and disrupt its structure, thereby enhancing reducing sugar release. It is a two-stage bioconversion process: hydrolysis of cellulose in corn stover to produce reducing sugars, and fermentation of the reducing sugars to bioethanol. In this study, the chemical compositions of the cellulase hydrolysate of corn stover pretreated by different methods were detected by HPLC in order to fred out the desirable pretreatment method. The results showed that the saccharification rate of CO2-1aser treatment combined with aeration and magnetic stir (LAM) was higher than those of other pretreatment methods.
Keywords:corn stover  pretreatment  enzymatic hydrolysate  HPLC
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