A new method for fast isolation of insect antifeedant compounds from complex mixtures |
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Authors: | Pierre Escoubas Yukiharu Fukushi Labunmi Lajide Junya Mizutani |
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Affiliation: | (1) Eniwa RBP, Research Development Corporation of Japan, Megumino Kita 3-1-1, Eniwa-Shi, 061-13 Hokkaido, Japan;(2) Department of Agricultural Chemistry Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan |
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Abstract: | Bioassay-guided isolation of bioactive natural substances requires monitoring of all fractionation and purification steps
using a bioassay system. This is often a long and tedious process, especially with insect feeding bioassays. We report here
a new method, based on the principle of bioautography, for a quick isolation of insect antifeedant compounds. TLC plates,
after development, are coated with a thin layer of artificial diet and fed toSpodoptera litura larvae. The location of uneaten areas is then compared with theR
fvalues of the TLC spots, in order to determine rapidly the active fractions. This methods allows for a very fast determination
of the most active antifeedant compounds in a complex mixture and considerably speeds up the isolation process. This new method
was successfully applied in the study of antifeedant activity of several plant samples, and results are presented here for
a model plant,Skimmia japonica (Rutaceae). Using this new method, the compounds responsible for the feeding-deterrent activity, three furanocoumarins (bergapten,
xanthotoxin, and oxypeucedanin), were quickly and efficiently identified. |
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Keywords: | Antifeedants tobacco cutworm Spodoptera litura Lepidoptera Noctuidae bioautography new bioassay Skimmia japonica Rutaceae furanocoumarins |
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