| 酶法提取茵陈总黄酮的工艺研究 |
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| 引用本文： | 李娟.酶法提取茵陈总黄酮的工艺研究[J].广州化工,2012,40(19):40-42. |
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| 作者姓名： | 李娟 |
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| 作者单位： | 洛阳理工学院环境工程与化学系,河南洛阳,471023 |
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| 基金项目： | 洛阳理工学院青年基金(2009QZ30) |
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| 摘 要： | 以酶法提取茵陈中黄酮类化合物,用紫外-可见比色法和芦丁为标准品测定茵陈总黄酮的得率,研究酶用量、pH值、温度、乙醇浓度、提取时间对黄酮得率的影响。最佳工艺条件为纤维素酶的浓度0.10 mg/mL,pH=4.2,温度50℃,乙醇浓度为60%,恒温振荡1.5 h,此条件下黄酮的得率为3.513%。
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| 关 键 词： | 茵陈 总黄酮 酶法 提取工艺 |
Study on Total Flavonids from Aremisia capillars Thunb. by Enzyme Method |
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| LI Juan.Study on Total Flavonids from Aremisia capillars Thunb. by Enzyme Method[J].GuangZhou Chemical Industry and Technology,2012,40(19):40-42. |
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| Authors: | LI Juan |
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| Affiliation: | LI Juan(Department of Enviromental and Chemical,Luoyang Institute of Science and Technology,Henan Luoyang 471023,China) |
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| Abstract: | Enzyme method was used to extract flavonoids compounds from Artemisia capillaris Thunb. .The content of total flavonoids in Artemisia capillaris Thunb. was determined by UV-spectrophotometry with standard troxerutin.The enzyme concentration,pH,temperature,ethanol concentration,extracting time of flavonoids were studied.The optimum extraction condition was the enzyme concentration of 0.1 mg/mL,pH of 4.2,tempreture of 50 ℃,the ethanol concentration of 60% and time of 1.5 h.The rate of extraction was 3.513%. |
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| Keywords: | Artemisia capillaris Thunb total flavonoids enzyme method extraction technique |
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