Genome-Wide Analysis of the Soybean Calmodulin-Binding Protein 60 Family and Identification of GmCBP60A-1 Responses to Drought and Salt Stresses |
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Authors: | Qian Yu Ya-Li Liu Guo-Zhong Sun Yuan-Xia Liu Jun Chen Yong-Bin Zhou Ming Chen You-Zhi Ma Zhao-Shi Xu Jin-Hao Lan |
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Affiliation: | 1.College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China; (Q.Y.); (Y.-L.L.); (Y.-X.L.);2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China; (G.-Z.S.); (J.C.); (Y.-B.Z.); (M.C.); (Y.-Z.M.) |
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Abstract: | Calmodulin-binding protein 60 (CBP60) members constitute a plant-specific protein family that plays an important role in plant growth and development. In the soybean genome, nineteen CBP60 members were identified and analyzed for their corresponding sequences and structures to explore their functions. Among GmCBP60A-1, which primarily locates in the cytomembrane, was significantly induced by drought and salt stresses. The overexpression of GmCBP60A-1 enhanced drought and salt tolerance in Arabidopsis, which showed better state in the germination of seeds and the root growth of seedlings. In the soybean hairy roots experiment, the overexpression of GmCBP60A-1 increased proline content, lowered water loss rate and malondialdehyde (MDA) content, all of which likely enhanced the drought and salt tolerance of soybean seedlings. Under stress conditions, drought and salt response-related genes showed significant differences in expression in hairy root soybean plants of GmCBP60A-1-overexpressing and hairy root soybean plants of RNAi. The present study identified GmCBP60A-1 as an important gene in response to salt and drought stresses based on the functional analysis of this gene and its potential underlying mechanisms in soybean stress-tolerance. |
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Keywords: | drought tolerance salt tolerance CBP60 proteins hairy root assay soybean Arabidopsis |
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