焦谷氨酸对高盐饮食小鼠肠道健康及肠道菌群的作用

建立高盐饮食小鼠模型,给予小鼠焦谷氨酸干预,通过对小鼠小肠病理学分析和形态学观察,检测炎性细胞因子、粪便中白蛋白渗漏量等相关物质,结合高通量测序分析小鼠盲肠内容物中菌群的α-多样性、β-多样性及基于门、属水平的物种组成,探究焦谷氨酸对高盐饮食小鼠肠道健康和肠道菌群的作用,开发其新应用方向。结果表明,焦谷氨酸可以显著缓解由高盐饮食引起的小鼠肠道结构损伤、降低小肠炎症细胞因子分泌水平。同时,焦谷氨酸可减少部分由高盐饮食导致显著增加的菌群丰度,例如拟杆菌属(Bacteroides)、另枝菌属(Alistipes)等;另使部分在高盐饮食过程中减少的菌群丰度得到恢复和部分益生菌丰度增加,包括分节丝状菌(Candidatus Arthromitus)、双歧杆菌属(Bifidobacterium)和乳酸杆菌属(Lactobacillus)等多种益生菌。综上,焦谷氨酸对高盐饮食小鼠机体显著的有益作用,可以稳定肠道结构、降低肠道炎症水平和改善肠道菌群结构和丰度。

Effects of pyroglutamic acid on intestinal health and intestinal microflora in mice with high-salt diet

In this experiment,a high-salt diet model was established in mice.The model mice were given pyroglutamic acid intervention to explore the effect of pyroglutamic acid on intestinal health and intestinal flora in mice with high-salt diet.Pathological analysis and morphological observation were performed on the small intestine of mice,followed by detection of intestinal inflammatory cytokines and albumin leakage in feces.Alpha diversity,beta diversity,and intestinal flora composition at the phylum and genus levels were analyzed by high-throughput sequencing.The results showed that pyroglutamic acid significantly alleviated the intestinal structural damage of mice caused by high-salt diet and reduced the secretion of inflammatory cytokines in small intestine.While pyroglutamic acid inhibited the significant increase in the abundance of some flora caused by high-salt diet,such as Bacteroides,Alistipes and other microorganisms.In addition,pyroglutamic acid also restored and increased the abundance of flora that had been reduced during high-salt diet,including Candidatus Arthromitus,Bifidobacterium,Lactobacillus and other probiotics.In summary,pyroglutamic acid has a significant beneficial effect on high-salt diet mice,which can stabilize the intestinal structure,reduce the level of intestinal inflammation,and improve the intestinal flora structure and abundance.