| 不同来源的霍乱弧菌毒力特征的质谱学分析 |
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| 引用本文： | 屠博文,薛银刚,姚萍,王楠,黎俊宏,唐宏兵,杜强. 不同来源的霍乱弧菌毒力特征的质谱学分析[J]. 食品工业科技, 2021, 42(6): 130-136,150. DOI: 10.13386/j.issn1002-0306.2020060092 |
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| 作者姓名： | 屠博文 薛银刚 姚萍 王楠 黎俊宏 唐宏兵 杜强 |
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| 作者单位： | 1. 常州市疾病预防控制中心, 江苏常州 213022;2. 常州大学环境与安全工程学院, 江苏常州 213003;3. 泰州医药高新区市场监督管理局医疗器械处, 江苏泰州 225300 |
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| 基金项目： | 江苏省卫生健康委科研课题面上项目(H2018098);常州市重大科技支撑计划(社会发展)(CE20185048和CE 20175022);江苏省预防医学会课题(Y2018023);常州市应用基础研究计划(CJ20190071)。 |
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| 摘 要： | 目的:分析本地区食品风险监测样品中霍乱弧菌的分布,评价使用VITEK-MS微生物质谱进行霍乱弧菌快速鉴定的优劣.方法 :从淡水产品和病人分离获得霍乱弧菌,分别进行VITEK-2生化鉴定和VITEK-MS质谱鉴定.血清凝集和胶体金显色法检测霍乱弧菌血清型,PCR检测ctx毒力基因.通过全基因组测序分析霍乱弧菌毒力基因类型...
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| 关 键 词： | 霍乱弧菌 微生物质谱(VITEK-MS) 全基因组测序 毒力基因 指纹图谱 |
| 收稿时间： | 2020-06-09 |
Virulence Characteristics Application of Vibrio cholera from Different Source by Microbial Mass Spectrometry |
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| TU Bowen,XUE Yingang,YAO Ping,WAND Nan,LI Junhong,TANG Hongbing,DU Qiang. Virulence Characteristics Application of Vibrio cholera from Different Source by Microbial Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(6): 130-136,150. DOI: 10.13386/j.issn1002-0306.2020060092 |
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| Authors: | TU Bowen XUE Yingang YAO Ping WAND Nan LI Junhong TANG Hongbing DU Qiang |
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| Affiliation: | 1. Changzhou Center of Disease Prevention and Control, Changzhou 213022, China;2. School of Environmental and Safety Engineering, Changzhou University, Changzhou 213003, China;3. Medical device department of market supervision administration of Taizhou pharmaceutical high-tech zone, Taizhou 225300, China |
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| Abstract: | Objective:To analysis the regularities of distribution of Vibrio cholera from food risk monitoring samples and evaluate the merits of VITEK-MS rapid analysis methods in identification of Vibrio cholera.Methods:V.cholera strains were isolated from freshwater products and patients.The VITEK-MS and VITEK-2 identification were operated respectively.The serotype was detected by serum agglutination and immunogold.The virulence genes ctxAB was detected by qPCR.All the virulence genes in different types of strains were analyzed by whole genome sequencing.Results:17 of the 23 V.cholera strains were non-O1/O139 serotype.6 strains were detected as O139 V.cholera and ctxAB gene carrier.All the V.cholera strain showed typical mass spectral characteristics based on the fingerprint spectrum.The particular and lacking peaks patterns of O139 V.cholera were representative for Serotype identification.ctxAB,ace and tcp islands genes were harbored in O139 Vibrio cholera,but only auxiliary virulence factor can be detected in non-O1/O139 Vibrio cholera except zot gene.Conclusion:There are many differences between the O139 and non-O1/O139 Vibrio cholera strains in whether virulence gene type or fingerprint spectrum.The thorough application for serotype identification by using microbial mass spectrometry may be a new assistance for cholera surveillance. |
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| Keywords: | Vibrio cholera microbial mass spectrometry(VITEK-MS) whole genome sequencing virulence genes fingerprint spectrum |
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