植物来源的黄酮类化合物抗体的制备和应用研究进展

黄酮类化合物是一类重要的天然产物。流行病学研究表明,黄酮类化合物可以降低罹患多种慢性病及癌症的风险,且对新冠肺炎COVID-19也有显著的辅助疗效。抗体是现代生命科学研究中极其重要的工具,黄酮类化合物的抗体在中医药及食品营养科学研究领域具有广阔的应用前景。该文简述了黄酮类化合物的药理作用,总结了国内外黄酮类化合物半抗原、完全抗原和抗体的制备进展,并对黄酮类化合物抗体的应用(主要包括酶联免疫分析、免疫亲和色谱方法、荧光免疫分析方法、免疫层析试纸条和Eastern blotting法)进行了阐述,为黄酮类化合物抗体的进一步研究提供参考。

Research progress on preparation and application of flavonoids antibody of plants

Flavonoids are important natural products.Epidemiological studies have shown that flavonoids can reduce the risk of a variety of chronic diseases and cancer,they also have a significant auxiliary effect on COVID-19.Antibodies are extremely important tools in modern life science research.Flavonoid antibody technology has broad application prospects in the fields of Chinese medicine and food nutrition science research.This article introduced the pharmacological effects of flavonoids and the research progress of the preparation of flavonoid haptens,complete antigen and antibodies.Besides,the application of flavonoid antibody which mainly includes enzyme-linked immunoassay,immunoaffinity chromatography method,fluorescence immunoassay method,immunochromatographic test strips and eastern blotting were illustrated,thus supporting the reference for further research of flavonoid antibodies.