蛹虫草丝瓜络半固态发酵产虫草素的工艺优化

采用丝瓜络作为蛹虫草孢子附着点和植物油吸附基质，经半固态发酵生产虫草素，旨在提高液体表面培养的比表面积，进一步提高虫草素产量。利用单因素和Box-Behnken试验设计分别对玉米油和橄榄油条件下的加液量、孢子接种量和发酵温度进行响应面优化。结果表明，虫草素最佳工艺条件为:植物油用玉米油，加液量为3.0 mL/g，孢子接种量为9.49%，发酵温度为27.3℃，相对湿度90%。在优化后的发酵条件下，虫草素产量达到10.13 g/L，比初始半固态发酵虫草素产量提高了76.48%。综上，发酵温度的控制是获得虫草素高产的关键因素。蛹虫草丝瓜络半固态发酵能够通过提高比表面积获得较高的虫草素产量，为大规模工业化虫草素生产提供理论支持。

Process Optimization of Semisolid Fermentation of Cordyceps militaris with Loofah for Cordycepin Production

Cordycepin was produced by semisolid fermentation of C.militaris using loofah as the attachment point of C.militaris spores and the adsorption matrix of vegetable oils in order to improve the specific surface area of liquid surface culture and further increase the cordycepin production.Single factor experiment and Box-Behnken experiment design were used to optimize the liquid content in loofah,spore inoculum size and fermentation temperature under the conditions of corn oil and olive oil by response surface methodology,respectively.The results showed that the optimum technological conditions of cordycepin were as follows:Corn oil as vegetable oil,liquid contents in loofah 3.0 mL/g,spore inoculum size 9.49%,fermentation temperature 27.3℃,relative humidity 90%.Under the optimized fermentation conditions,the cordycepin production reached 10.13 g/L,which was 76.48%higher than the initial semisolid fermentation.It suggested that,the control of fermentation temperature was the key factor to obtain high cordycepin production.Semisolid fermentation of C.militaris with loofah could obtain higher cordycepin accumulation by increasing specific surface area,which could provide theoretical support for large-scale industrial production of cordycepin.