| 好食脉孢霉发酵产类胡萝卜素的鉴定、抗氧化性及稳定性研究 |
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| 引用本文： | 邓永平,车鑫,艾瑞波,刘晓兰,辛嘉英,王晓杰.好食脉孢霉发酵产类胡萝卜素的鉴定、抗氧化性及稳定性研究[J].食品与发酵工业,2021(4):15-20. |
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| 作者姓名： | 邓永平 车鑫 艾瑞波 刘晓兰 辛嘉英 王晓杰 |
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| 作者单位： | 齐齐哈尔大学食品与生物工程学院;黑龙江省玉米深加工理论与技术重点实验室;哈尔滨商业大学 |
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| 基金项目： | 黑龙江省省属高等学校基本科研业务费科研项目“粮头食尾”专项(LTSW201740);黑龙江省省属高等学校基本科研业务费科研项目校地合作项目(135409507);黑龙江省自然科学基金项目(LH2020C063)。 |
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| 摘 要： | 利用液相色谱-质谱联用技术对好食脉孢霉(Neurospora sitophila)固态发酵生产的类胡萝卜素进行初步鉴定,研究了类胡萝卜素粗提物的抗氧化活性和稳定性。N.sitophila来源的类胡萝卜素的主要成分之一为β-胡萝卜素;当质量浓度为6μg/mL时,类胡萝卜素的DPPH自由基清除率显著高于α-生育酚、丁基羟基茴香醚(butyl hydroxylanisole,BHA)和β-胡萝卜素(P<0.05),羟自由基清除率显著高于α-生育酚、BHA、特丁基对苯二酚(tert-butyl hydroquinone,TBHQ)和β-胡萝卜素(P<0.05),总还原力显著高于α-生育酚和β-胡萝卜素(P<0.05);类胡萝卜素在避光、低温及不含K^+、Na^+、Mg^2+、Ca^2+、Ba^2+、Cu^2+、Fe^3+的条件下较稳定。研究结果表明好食脉孢霉类胡萝卜素具有较强的抗氧化活性,具有开发为天然抗氧化剂的巨大潜力。
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| 关 键 词： | 好食脉孢霉 类胡萝卜素 液相色谱-质谱技术 抗氧化活性 |
Identification,antioxidant and stability of carotenoids from a GRAS fungus Neurospora sitophila |
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| DENG Yongping,CHE Xin,AI Ruibo,LIU Xiaolan,XIN Jiaying,WANG Xiaojie.Identification,antioxidant and stability of carotenoids from a GRAS fungus Neurospora sitophila[J].Food and Fermentation Industries,2021(4):15-20. |
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| Authors: | DENG Yongping CHE Xin AI Ruibo LIU Xiaolan XIN Jiaying WANG Xiaojie |
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| Affiliation: | (College of Food and Biological Engineering,Qiqihar University,Qiqihar 161006,China;Key Laboratory of Corn Deep Proessing Theory and Techology,Qiqihar University,Qiqihar 161006,China;Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150076,China) |
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| Abstract: | A liquid chromatography-mass spectrometry was developed for preliminary identification of carotenoids produced by Neurospora sitophila through solid-state fermentation,then antioxidant activity and stability of carotenoids were investigated.One of the main components of carotenoids from N.sitophila wasβ-carotene.When the carotenoid concentration was 6μg/mL,the DPPH·clearance rate of carotenoids was significantly higher than that ofα-tocopherol,butyl hydroxylanisole(BHA)andβ-carotene(P<0.05);the·OH clearance rate was significantly higher than that ofα-tocopherol,BHA,tert-butyl hydroquinone(TBHQ)andβ-carotene(P<0.05);the total reduction capacity of carotenoids was significantly higher than that ofα-tocopherol andβ-carotene(P<0.05).Carotenoids were relatively stable under the conditions of avoiding light,low temperature and free of some ions such as K^+,Na^+,Mg ^2+,Ca ^2+,Ba ^2+,Cu ^2+and Fe^ 3+.The above results showed that carotenoids from N.sitophila have excellent antioxidant activities,indicating great potential as natural antioxidants. |
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| Keywords: | Neurospora Sitophila carotenoids liquid chromatography-mass spectrometry technology antioxidant activity |
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