磷酸三钙陶瓷人工骨的细胞相容性研究

将磷酸三钙（ＴＣＰ）陶瓷与Ｌ９２９细胞体外混合培养，ＭＴＴ法检测显示细胞增殖活性未受抑制；ＴＣＰ陶瓷与成骨细胞体外混合培养，扫描电镜观察见细胞长满培养孔底玻片上，其生长行为与单纯成骨细胞组无区别；ＴＣＰ陶瓷与骨髓细胞体外混合培养，倒置相差显微镜观察见大量骨髓细胞生长贴附于材料表面、孔道内及培养孔底，其生长行为与单纯骨髓细胞组无差异。这些结果表明ＴＣＰ陶瓷无细胞毒性，具有良好的细胞相容性。

Study on Cytocompatibility of Tricalcium Phasphate Ceramics Artificial Bone

When L929 cells mixed with tricalicuim phosphate (TCP) ceramics were cultured in vitro,their growth was not inhibited in analysis of MTT method;when osteoblasts mixed with TCP ceramics were cultured in vitro,they grown well and spread entensively over coverslip,and no significant difference on their growth was noted comparing with osteoblasts group alone under SEM observation;when marrow cells mixed with TCP ceramics were cultured in vitro,a large amount of cells adhered well to the surface and inner wall of TCP creamies and spread well over the dish bottom,and no significant difference on their growth was noted comparing with marrow cells group alone under inversive phase contrast microscope observation. These reults demonstrated that the TCP ceramics did not show cytotoxicity and possessed an excellent cytocompatibity.