基因表达谱芯片用于小鼠膝关节炎组织的检测与分析

通过副韧带及半月板切除方法(MLI-OA)建立小鼠KOA模型,补肾活血方灌胃给药、常规抽提和纯化RNA,采用Agilent SurePrint G3 Mouse GE V2.0基因表达谱芯片检测小鼠膝OA关节组织的基因表达变化。检测结果显示补肾活血方对KOA组织基因表达具有明显调控作用补肾活血方有明显上调作用的基因中,中药组比模型组上调2倍以上的有56个;明显下调作用的基因中,中药组比模型组下调2倍以上的有119个。研究结果表明补肾活血方可能通过调控目前在KOA病变过程中作用尚不明确的基因,促进关节炎性因子代谢,延缓软骨退变,治疗骨关节炎的作用。

Detection and analysis of knee osteoarthritis in mice with gene expression profile

When gene expression profile is used for gene detection,the probe on the chip can emit fluorescence with different wavelengths.Under the action of confocal laser scanner,a clear gene change image can be obtained,by which the gene changes of the sample to be tested can be observed directly.First,the knee osteoarthritis (KOA)models of mice are established by the method of collateral ligament and meniscus resection (MLI-OA).Then,Bushen Huoxue formula is given by gavage,and ribonucleic acid (RNA)is routinely extracted and purified.Finally,the gene expression changes of KOA tissues of mice are detected by Agilent SurePrint G3 Mouse GE V2.0 gene expression profile.The results show that Bushen Huoxue formula has significant regulation effect on gene expression of KOA tissue.Among the genes with significant up-regulation effect of Bushen Huoxue formula,there are 56 genes of traditional Chinese medicine (TCM)groups up-regulated more than twice compared with model groups.Among the genes with significant down-regulation effect,there are 119 genes of TCM groups down-regulated more than twice compared with model groups.The experimental results indicate that Bushen Huoxue formula may promote the metabolism of arthritic factors and delay cartilage degeneration to treat KOA by regulating genes that are currently unknown in the pathological process of KOA.