线粒体ATP合成酶复合体分离纯化的方法学研究与探讨

科研工作者们在过去的50年前赴后继的工作中深入研究了ATP合成酶的功能,并努力尝试解析该酶的空间结构以其能够从结构基础上对ATP合成酶的催化机理进行阐明;但蛋白的纯化工作却一直是困扰研究顺利进展的最大障碍.蛋白的纯化技术是与特定阶段科技发展及科研工作者思维模式的直接反应,因而是一门不断发展的艺术.高纯度的ATP合成酶及其亚基是研究酶结构与催化机理的基础和关键,文章综合半个世纪以来ATP合成酶研究发展,提出了一条较为完备的ATP合成酶提取与纯化的方法路线,并对FO-ATPase部分亚基c的纯化方法进行了重点介绍.

Discussion and Practice on the Improved Methods for Isolation and Purification of Mitochondria ATP Synthase Complex

In the past 50 years,scientists have done so much on ATP synthase's structure and its function alreadv,but people known function more and the mechanism fewer,and there are much more work need to do.High purified ATPase and subunits which should come firstly are the key factors in study of enzyme's structure and catalvtic mechanism.This review has summarized some valuable methods in extraction and purification of ATP synthase through compared with kinds of methods came from different workgroups all of the world,and mainly introduced the isolation of bovine heart mitochondria.submichondrial particles,F1-ATPase.F0-AIPase and their subunits such as a,b,g,d,e in F1-ATPase and b,c,d,e,f,g,h,F6,A6L in FO moiety and oligomycin sensitive conferring ATPase (OSCP) and the ATPase inhibitor (IF1) in details.