UPLC法测定绿豆中牡荆苷与异牡荆苷含量

建立了同时测定绿豆中牡荆苷与异牡荆苷含量方法:采用美国Waters ACQUITY UPLC系统,ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱;流动相:甲醇-0.5%冰醋酸溶液(20∶80);检测波长:338 nm;柱温:30℃;流速:1 mL/min;进样量:1μL。结果表明:该含量测定方法的精密度、稳定性、重复性、准确度良好,牡荆苷在0.685～6.85μg/mL,异牡荆苷在0.645～6.45μg/mL线性关系良好。三批样品中牡荆苷与异牡荆苷的平均含量为0.0467%和0.0555%。赤豆及黑豆中未检测到牡荆苷与异牡荆苷。所建立的含量测定方法快速、简便、准确,为绿豆解毒功效的物质基础研究提供质量控制方法。

Simultaneous Determination of Vitexin and Isovitexin in Mung Bean by UPLC

The UPLC method was established for simultaneous determination of vitexin and isovitexin in mung bean.The separation was achieved on a C18 column（2.1 mm×50 mm,1.7 μm） with a mobile phase consisting of methanol-0.5% acetic acid（20：80）.The flow rate was 1 mL/min.The column temperature was maintained at 30 ℃.The detection wavelength was at 338 nm.The precision,stability and repeatability of the method were good.The linear range of vitexin and isovitexin was 0.685~6.85 μg/mL and 0.645~6.45 μg/mL,respectively.The average content of three batch samples was 0.0467% for vitexin and 0.0555% for isovitexin.The method was accurate and repeatable,which was better for quality evaluation of vitexin and isovitexin in mung bean.