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橙皮苷分析方法研究
引用本文:贺丽娜,叶华,郭方遒. 橙皮苷分析方法研究[J]. 广州化工, 2012, 40(14): 126-128
作者姓名:贺丽娜  叶华  郭方遒
作者单位:中南大学化学化工学院,湖南长沙,410083
摘    要:以橙皮苷为标准品,采用紫外分光光度法(直接法)和高效液相色谱(HPLC)测定橙皮苷含量,并对测定条件进行探讨,比较两种方法测定橙皮苷的准确度。实验结果表明,紫外分光光度法测得的橙皮苷纯度数据比高效液相色谱数据高,HPLC法精确,分光光度法简单快速,均能得到令人满意的结果。

关 键 词:橙皮苷  紫外分光光度法  高效液相色谱法

Study on Analytical Method of Hesperidin
HE Li-na,YE Hua,GUO Fang-qiu. Study on Analytical Method of Hesperidin[J]. GuangZhou Chemical Industry and Technology, 2012, 40(14): 126-128
Authors:HE Li-na  YE Hua  GUO Fang-qiu
Affiliation:HE Li - ha, YE Hua , GUO Fang - qiu (School of Chemistry and Chemical Engineering, Central South University, Hunan Changsha 410083, China)
Abstract:Hesperidin as standard sample, the content of hesperidin was determined with UV - Vis spectrophotometry method and high performance liquid chromoma to graphy (HPLC), and the determine conditions were discussed. Mean- while, compared with these two determination methods, the accuracies were compared. The results showed that the purity of hesperidin analyzed by UV - Vis spectrophotometry method was marginally higher than that of HPLC. UV - Vis spectro- photometry method was simple and convenient, while HPLC can get high accuracy result.
Keywords:hesperidin  UV - Vis spectrophotometry method  HPLC
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