HPLC法测定复方板蓝根口服液中表告依春的含量

建立了高效液相色谱法测定复方板蓝根口服液中表告依春的含量。采用高效液相色谱法。色谱柱为Aigleng C18柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.01 mol/L磷酸二氢钠缓冲液(氢氧化钠试液调节pH 7.2)(7∶93)流速:1.0 mL/min,检测波长240 nm;柱温:30℃。结果显示:表告依春色谱峰分离良好,表告依春进样量在0.01938～0.3876μg范围内线性关系良好,回归方程:A=7154.3C-22.73,r=0.9999;平均回收率为99.7%,RSD为0.77%(n=6)。该测定方法简便,准确,重现性好,精度高,可用于该制剂的质量控制。

HPLC for Determining the Content of Epigoitrin in Compound Banlangen Oral Solution

HPLC for determining the content of Epigoitrin in Compound Banlangen Oral Solution was established. Stationary phase was Aigleng C18 chromatographic column （4.6 ×250mm, 5μm）, mobile phase was methanol -0.01 mol/L sodium dihydrogen phosphate buffer solution （ Adijusted to pH 7.2 with sodium hydroxide solution） （ 7 ： 93 ）, de- tection wavelength was 240 nm, flow rate was 1.0 mL/min and column temperature was 30℃. Epigoitrin had a good separation of peaks, the calibration curve were linear over the range of 0.01938 -0.3876 μg, and the regression equation was A = 7154.3C - 22.73, r = 0.9999. The average recovery was 99.7% with RSD = 0.77% （n = 6）. This method was simple, accurate, with good reproducibility and high precision, and can be used to control the quality of Compound Ban- langen Oral Solution.