蒲公英糖蛋白的含量测定及两种提取工艺优化

采用单因子试验和L9(34)正交试验设计,比较了热水浸提、超声波辅助热水浸提中料液比、提取时间、提取温度、盐浓度和超声波功率对蒲公英糖蛋白提取率的影响。热水浸提蒲公英糖蛋白的最佳工艺参数为:料液比1∶25、温度80℃,浸提时间3 h、盐浓度0.1 mol/L。超声波辅助提取的最佳工艺参数为:料液比1∶15、功率120 W、时间15 min、盐浓度0.1 mol/L。两种方法相比,超声波辅助法所需时间更短,但糖蛋白提取率较热水浸提低,在实际应用时需要综合考虑。

Study of Quantitative Determination Method and Optimizing Conditions for Glycoprotein Extraction from Dandelion

The method for quantitative determination of glycoprotein from dandelion was studied and the extraction condition were optimized.The optimization strategy used was single factor test and orthogonal design test of L9（34）to study the effects of the extraction temperature,time,the ratio of solid to liquid,the salt concentration and ultrasonic power by ultrasonic assists extraction method and hot water extraction method.The optimal extraction temperature,time,liquid-to-solid ratio,the salt concentration and ultrasonic power were 80 ℃,3 h,1：25,0.1 mol/L,respectively,using hot water extraction method.And the optimal ultrasonic power,ultrasonic treatment time,liquid-to-solid ratio,the salt concentration were 120 W,15 min,1：15,0.1 mol/L,respectively,using ultrasonic assists method.A comparative study demonstrated that the hot water method taken longer time and higher extraction rate.So it should be taken various elements into the comprehensive consideration when actual application.