高效液相色谱法测定食用植物油中的游离棉酚

研究采用高效液相色谱法测定食用植物油中游离棉酚的方法,最佳色谱条件:色谱柱C18柱,流动相为甲醇-磷酸溶液(1%)=84+16,波长240 nm,流速为1.0 mL/min,柱温30℃。曲线方程y=3.1033×10-5x,相关系数r=0.999871,线性关系良好。检出限为2.5 mg/kg,回收率为98.0%～100.2%。该法简便,快速,准确性高,重现性好。

Determination of Free Gossypol in Edible Vegetable Oil by HPLC

The method of determination that free gossypol in edible vegetable oil was established by using HPLC.The best chromatographic conditions were as follows： the column of chromatogram C18,mobile phase methanol-phosphate buffer（1%）=84 ＋16,U V-240 nm detector,flow rate 1.0 mL/min,and column temperature 30 ℃.Linear regression equation y=3.1033×10-5x,the correlation coefficient r=0.999871,and linear relationship was satisfied.The detection limit was 2.5 mg/kg,and recovery was 98.0~100.2%.The method was simple,fast,high accuracy and good reproducibility.