| 高效液相色谱法测定食用植物油中的游离棉酚 |
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| 引用本文： | 张庆珍. 高效液相色谱法测定食用植物油中的游离棉酚[J]. 广州化工, 2012, 40(15): 159-160 |
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| 作者姓名： | 张庆珍 |
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| 作者单位： | 肇庆市质量计量监督检测所,广东肇庆,526060 |
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| 摘 要： | 研究采用高效液相色谱法测定食用植物油中游离棉酚的方法,最佳色谱条件:色谱柱C18柱,流动相为甲醇-磷酸溶液(1%)=84+16,波长240 nm,流速为1.0 mL/min,柱温30℃。曲线方程y=3.1033×10-5x,相关系数r=0.999871,线性关系良好。检出限为2.5 mg/kg,回收率为98.0%～100.2%。该法简便,快速,准确性高,重现性好。
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| 关 键 词： | 高效液相 植物油 游离棉酚 |
Determination of Free Gossypol in Edible Vegetable Oil by HPLC |
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| ZHANG Qing-zhen. Determination of Free Gossypol in Edible Vegetable Oil by HPLC[J]. GuangZhou Chemical Industry and Technology, 2012, 40(15): 159-160 |
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| Authors: | ZHANG Qing-zhen |
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| Affiliation: | ZHANG Qing-zhen(Institute of Quality Supervision and Inspection,Guangdong Zhaoqing,526060,china) |
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| Abstract: | The method of determination that free gossypol in edible vegetable oil was established by using HPLC.The best chromatographic conditions were as follows： the column of chromatogram C18,mobile phase methanol-phosphate buffer（1%）=84 ＋16,U V-240 nm detector,flow rate 1.0 mL/min,and column temperature 30 ℃.Linear regression equation y=3.1033×10-5x,the correlation coefficient r=0.999871,and linear relationship was satisfied.The detection limit was 2.5 mg/kg,and recovery was 98.0~100.2%.The method was simple,fast,high accuracy and good reproducibility. |
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| Keywords: | HPLC edible oil free gossypol |
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