Fine Mapping and Gene Analysis of restorer-of-fertility Gene CaRfHZ in Pepper (Capsicum annuum L.) |
| |
| Authors: | Zhixing Nie Yunpeng Song Hong Wang Jianying Chen Qingliang Niu Weimin Zhu |
| |
| Affiliation: | 1.Shanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; (Z.N.); (Y.S.);2.Vegetable Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; (H.W.); (J.C.);3.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China |
| |
| Abstract: | Cytoplasmic male sterility (CMS) is a common biological phenomenon used in hybrid production of peppers (Capsicum annuum L.). Although several restorer-of-fertility (Rf) genes of pepper CMS lines have been mapped, there is no report that the Rf gene with clear gene function has been isolated. Here, pepper CMS line HZ1A and its restorer line HZ1C were used to construct (HZ1A × HZ1C) F2 populations and map the Rf gene. A single dominant gene CaRfHZ conferred male fertility according to inheritance analysis. Using sterile plants from (HZ1A × HZ1C) F2 populations and bulked segregant analysis (BSA), the CaRfHZ gene was mapped between P06gInDel-66 and P06gInDel-89 on chromosome 6. This region spans 533.81 kb, where four genes are annotated according to Zunla-1 V2.0 gene models. Based on the analysis of genomic DNA sequences, gene expressions, and protein structures, Capana06g002968 was proposed as the strongest candidate for the CaRfHZ gene. Our results may help with hybrid pepper breeding and to elucidate the mechanism of male fertility restoration in peppers. |
| |
| Keywords: | pepper cytoplasmic male sterility restorer-of-fertility gene fine mapping |
|
|
