Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action |
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| Authors: | Xiaoyan Hou Jianlong Li Huaqiao Tang Qingye Li Guanghui Shen Shanshan Li Anjun Chen Zixin Peng Yu Zhang Chaowei Li Zhiqing Zhang |
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| Affiliation: | 1.College of Food Science, Sichuan Agricultural University, Ya’an 625014, China; (X.H.); (J.L.); (Q.L.); (G.S.); (S.L.); (A.C.); (Z.P.); (Y.Z.); (C.L.);2.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611137, China; |
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| Abstract: | Our previous study extracted and identified an antibacterial peptide that was named NP-6. Herein, we investigated the physicochemical properties of NP-6, and elucidated the mechanisms underlying its antimicrobial activity against Staphylococcus aureus. The results showed that the hemolysis activity of NP-6 was 2.39 ± 0.13%, lower than Nisin A (3.91 ± 0.43%) at the same concentration (512 µg/mL). Negligible cytotoxicity towards RAW264.7 cells was found when the concentration of NP-6 was lower than 512 µg/mL. In addition, it could keep most of its activity in fetal bovine serum. Moreover, transmission electron microscopy, confocal laser scanning microscopy, and flow cytometry results showed that NP-6 can destroy the integrity of the bacterial cell membrane and increase the membrane permeability. Meanwhile, NP-6 had binding activity with bacterial DNA and RNA in vitro and strongly inhibited the intracellular β-galactosidase activity of S. aureus. Our findings suggest that NP-6 could be a promising candidate against S. aureus. |
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| Keywords: | antibacterial peptide Staphylococcus aureus modes of action |
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