GASA基因超家族新成员——烟草抗菌肽基因的克隆及序列分析

抗菌肽可以增强植物对病原微生物的抵抗能力,本研究利用生物信息学方法,获得了烟草品种K326的3种抗菌肽cDNA序列(NtSN1a、NtSN16和NtSN2a),并对其进行了序列分析.结果表明,3种抗茵肽cDNA序列均具有完整的开放读码框,NtSNIa和NtSNIb均编码88个氨基酸,NtSN2a编码104个氨基酸.对推导的氨基酸序列分析发现,它们都具有典型的GASA基因超家族保守结构域,属于GASA基因超家族成员.系统进化分析表明,NtSN1a和NtSN16与马铃薯抗菌肽snaking-1聚为一类,NtSN2a与马铃薯抗菌肽snaking-2聚为一类.这些结果为进一步研究抗菌肽基因的抑菌效应,比较其在烟草抗病防御反应中的作用奠定了基础.

Cloning and sequence analysis of tobacco antimicrobial peptide genes,novel members of GASA gene superfamily

Antimicrobial peptides(AMPs) have been considered to play a key role in plant defense.Three distinct AMP cDNAs(NtSN1a,NtSN1b and NtSN2a) were cloned from tobacco cultivar K326 using in silicon cloning technique,and were analyzed by bioinformatics method.Sequence analysis revealed that both NtSN1a and NtSN1b encoded an open-reading frame of 88 amino acids,and NtSN2a encoded an open-reading frame of 104 amino acids.,all of them shared the conserved domain of the GASA superfamily.Phylogenetic analysis showed that NtSN1a and NtSN1b cluster with potato snaking-1,and NtSN2a clusters with potato snaking-2.These results lay the foundation for clarifying the inhibitory effect of AMPs,discovering their functional differences and understanding the molecular mechanisms underlying tobacco defense responses.