Use of Hydration, Germination, and α-Galactosidase Treatments to Reduce Oligosaccharides in Dry Beans |
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Authors: | N. J. Matella K.D. Dolan A. W. Stoeckle M. R. Bennink Y. S. Lee M. A. Uebersax |
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Affiliation: | Authors Matella, Dolan, Bennink, Lee and Uebersax are with Dept. of Food Science and Human Nutrition, Michigan State Univ., G.M. Trout Bldg., East Lansing, MI 48824.;Authors Dolan and Stoeckle are with Dept. of Biosystems and Agricultural Engineering, Michigan State Univ., East Lansing, Mich. Direct inquiries to author Dolan (E-mail: ). |
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Abstract: | ABSTRACT: This investigation was done to determine an energetically feasible method to reduce bean oligosaccharides (stachyose and raffinose) for large-scale bean flour production. Michigan black, red, and navy beans (all Phaseolus vulgaris ) were treated by α-galactosidase, germination, or hydration to determine treatment effects on stachyose and raffinose levels. Oligosaccharides were extracted with 70% (w/w) ethanol, and α-galactosidase was added to catalyze hydrolysis of the oligosaccharides. Enzyme treatment was for 1 h at ambient temperature (23°C). For germination, whole beans were incubated in a germinating chamber (100% humidity, 26.7°C) for 48 h. In hydration, whole beans were soaked in excess water at ambient temperature (23°C) for 5 h. Enzyme treatment resulted in 51%± 3.0%, 30%± 1.9%, and 46%± 1.9% reduction in total raffinose and stachyose for black beans, red beans, and navy beans, respectively. Germination treatment resulted in 61%± 12%, 61%± 1.9%, and 70%± 2.8% reduction in total raffinose and stachyose for black beans, red beans, and navy beans, respectively. Hydrated black beans showed a loss of 33%± 9.6%, whereas hydrated red and navy beans showed no reduction of oligosaccharides. Although germination showed the greatest reduction in bean oligosaccharides, this method may not be as cost-effective commercially as the enzyme treatment because of the time required for germination. |
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Keywords: | dry beans oligosaccharide reduction hydration germination α-galactosidase |
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