Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method |
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| Authors: | X. MENG X. TIAN Y. KONG A. SUN W. YU W. QIAN X. SONG H. CUI L. XUE C. LIU S. WANG |
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| Affiliation: | 1. Department of Optoelectronic Information Science and Engineering, School of Science, Jiangnan University, Wuxi, Jiangsu, China;2. Present Address: Advanced Photonics Research Center, Laser Institute of Shandong Academy of Sciences, Qingdao, Shandong, China;3. College of Electronics and Information Engineering, Shanghai University of Electric Power, Shanghai, China |
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| Abstract: | Transport of intensity equation (TIE) method can acquire sample phase distributions with high speed and accuracy, offering another perspective for cellular observations and measurements. However, caused by incorrect focal plane determination, blurs and halos are induced, decreasing resolution and accuracy in both retrieved amplitude and phase information. In order to obtain high‐accurate sample details, we propose TIE based in‐focus correction technique for quantitative amplitude and phase imaging, which can locate focal plane and then retrieve both in‐focus intensity and phase distributions combining with numerical wavefront extraction and propagation as well as physical image recorder translation. Certified by both numerical simulations and practical measurements, it is believed the proposed method not only captures high‐accurate in‐focus sample information, but also provides a potential way for fast autofocusing in microscopic system. |
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| Keywords: | In‐focus corrections quantitative phase imaging transport of intensity equation |
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