东北山核桃仁油对D-半乳糖衰老小鼠抗氧化能力的影响

研究东北山核桃仁油(Juglans mandshurica Oil,JMO)对D-半乳糖衰老小鼠抗氧化能力的影响。采用连续颈背部注射D-半乳糖建小鼠亚急性衰老模型的方法,造模6周。建模同时,以VC为阳性物质对照组,JMO低、中、高剂量组分别灌胃5、10、20mL/(kg.d),同时设模型对照组,灌胃生理盐水。6周后测定小鼠血清、肝脏、脑组织中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和单胺氧化酶(MAO)的含量。结果表明:模型对照组血清、肝脏和脑组织匀浆中T-AOC、S OD、C A T、G S H-P x含量均显著降低,M AO和M D A含量显著升高,与正常对照组比较差异显著;1 0、2 0mL/(kg.d)中、高剂量JMO可显著提高衰老小鼠机体总抗氧化能力T-AOC水平,显著提高抗氧化酶SOD、CAT和GSH-Px的活性,明显降低MAO活性和脂质过氧化物MDA含量,与模型对照组比较有显著性差异。提示,JMO能抑制机体内脂质过氧化物的生成,有效清除机体内产生的过量自由基,显著提高亚急性衰老小鼠的抗氧化能力,具有量效关系。

Effect of Oil from Juglans mandshurica Grown in Northeast China on Antioxidant Abilities in D-Galactose-Induced Aging Mice

To investigate the influence of Juglans mandshurica Oil(JMO) on antioxidant abilities in aged mice,a subacute aging mice model was built by neck back subcutaneous injection of D-galactose for 6 consecutive weeks.Using VC as positive control,JMO was given to mice by gavage at three different doses of 5,10 mL/(kg.d) and 20 mL/(kg.d).The control and model control groups were given normal saline by gavage during that time.Total antioxidant capacity(T-AOC),catalase(CAT),malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and monoamine oxidase(MAO) in the serum,liver and brain of mice were determined at the end of the last administration.The results showed that the content of T-AOC,SOD,CAT and GSH-Px in the aged model group significantly declined(P <0.05),but the content of MAO and MDA significantly increased as compared to the normal control group(P < 0.05).For the aged mice treated with 10 mL(kg.d)and 20 mL/(kg.d) of JMO,the activities of SOD,CAT,GSH-Px and the content of T-AOC significantly increased(P <0.05) and the activity of MAO and the content of MDA significantly declined(P < 0.05) as compared to aged control mice.These data indicated that JMO could inhibit the generation of lipid peroxides,and effectively remove free radicals,and significantly increase antioxidant ability in the body in a dose-dependent manner.