用于液相检测的双参数压电DNA传感器研究 |
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引用本文: | 高志贤,晁福寰,王红勇,房彦军,潘海峰,朱惠忠. 用于液相检测的双参数压电DNA传感器研究[J]. 传感器与微系统, 2003, 22(10): 78-80 |
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作者姓名: | 高志贤 晁福寰 王红勇 房彦军 潘海峰 朱惠忠 |
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作者单位: | 1. 军事医学科学院,卫生学环境医学研究所,天津,300050 2. 清华大学,精密仪器与机械学系,北京,100084 |
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基金项目: | 国家自然科学基金(39970644,30271159),天津市自然科学基金(00360811) |
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摘 要: | 采用自组装技术,利用亲和素-生物素系统将25-mer的生物素标记的DNA探针固定于石英谐振器金电极上,与双参数压电传感器相结合,研制成了用于液相检测的压电DNA传感器。该传感器稳定性较好,3h频移在5Hz以内;液体的体积在一定范围内对双参数传感器未见明显影响;用双参数方法制作的传感器特异性等性能较好,为实现检测的自动化和生物的动力学测定打下了基础。
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关 键 词: | 压电DNA传感器 分子自组装膜 液相检测 基因探针 |
文章编号: | 1000-9787(2003)10-0078-03 |
修稿时间: | 2003-04-23 |
Study of dual parameter piezoelectric DNA sensor for liquid detection |
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Abstract: | Piezoelectric DNA sensor for liquid detection is developed with molecular self assembled monolayers technique that 25 mer biotinylted oligonucleotides are immobilized on gold electrode surface of quartz crystal with avidin biotin system and combined with dual parameter piezoelectric sensor.The performance of the sensor is better, such as frequence shift is 5?Hz in 3h,non specific hybridized reaction of the sensor.Liquid volume in a certain range has no effect on the sensor.Those studies provide stable base for automatization of the sensor and biomaterial kinetic detection. |
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Keywords: | piezoelectric DNA sensor molecular self-assembled monolayers liquid detection gene probe |
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