2-APB通过下调TRPM7在小鼠皮肤创伤愈合中的作用

目的：探究2-氨基乙基二苯基硼酸酯（2-APB）对小鼠皮肤创伤愈合的促进作用及其可能的机制。 方法：将实验小鼠分为5组：Control组、二甲基亚砜（DMSO）组、低（50 mg/L）、中（100 mg/L）和高（200 mg/L）浓度2-APB组，在每只小鼠皮肤背部脊正中线两侧1 cm左右用圆形打孔器各打一个直径10 mm、深至皮下的皮肤创口，其中Control组仅用纱布包扎，不敷用各种药剂；DMSO组每天敷1 g DMSO/凡士林膏剂；2-APB组每天敷1 g相对应浓度的2-APB/凡士林膏剂，隔天拍照观察愈合情况，并于第21天取材，使用HE染色观察创面病理形态以及Western blot检测TRPM7、转化生长因子-β（TGF-β）、I型胶原（Collgen-I, Col-I）以及IL-1β表达情况。结果：与Control组和DMSO组相比，不同浓度的2-APB均可显著促进小鼠皮肤伤口愈合（P<0.01），而DMSO组与Control组之间创伤愈合率无明显差异。HE染色结果显示，与Control组和DMSO组相比，2-APB可增加创面胶原含量以及真皮层的厚度（P<0.01），而DMSO组与Control组之间无明显差异。同时，2-APB还可显著增加创面TGF-β和Col-I的表达，抑制TRPM7和IL-1β的表达。 结论：不同浓度2-APB（50、100和200 mg/L）对皮肤创伤愈合有促进作用，其机制可能与抑制TRPM7有关。

Effects of 2-APB in skin wound healing in mice through down-regulation of TRPM7

AIM: To explore the promoting effect of 2-APB on skin wound healing in mice and its potential mechanism. METHODS: KM mice were divided into 5 groups: control group, DMSO group, low (50 mg/L), medium (100 mg/L) and high (200 mg/L) concentration 2-APB group. On the back of each mouse's skin use a circular punch about 1 cm on both sides of the midline of the spine to make a skin wound with a diameter of 10 mm and as deep as the fascia. The control group was only wrapped with gauze and no drugs were applied; the DMSO group was applied 1 g DMSO/Vaseline ointment per day; in the 2-APB group, apply 1 g of 2-APB/Vaseline ointment at a corresponding concentration every day. Pictures were taken the next day to observe the healing, and the material was taken on the 21st day, HE staining was used to observe the pathological morphology of the wound and western blot to detect TRPM7, TGF-β, collagen-I and IL-1β expression. RESULTS: Compared with the control group and the DMSO group, different concentrations of 2-APB could significantly promote skin wound healing in mice (P<0.01), but there was no significant difference in wound healing rate between the DMSO group and the control group group. The results of HE staining showed that, compared with the control group group and the DMSO group, 2-APB could increase the collagen content of the wound and the thickness of the dermis (P<0.01), but there was no significant difference between the DMSO group and the control group group. At the same time, 2-APB could also significantly increase the expression of TGF-β and Col-I on the wound, and inhibit the expression of TRPM7 and IL-1β. CONCLUSION: Different concentrations of 2-APB (50, 100 and 200 mg/L) can promote skin wound healing, and its mechanism may be related to the inhibition of TRPM7.