The method of eliminating phenol with the use of tyrosinase Agaricus bisporus immobilized in alginate

We have considered the method of oxidizing aqueous solutions of phenol (0.25–2.00 mmol/dm³) with the use of a biocatalyst—a partially purified tyrosinase immobilized into calcium alginate. In optimal conditions (pH 7.2, T—25°C, transformation time—one hour, phenoloxidase activity—25–200 unit/cm³) when adding a PEG-6000 stabilizer the preparation stabilized complete oxidation of phenol in a periodic-action reactor during 12 cycles and retained a high degree of transformation of the substrate during the following 16 cycles. The concentrations of coagulants necessary for elimination of transformation products using the immobilized enzyme were reduced 2.5-fold compared with such for a free biocatalyst.