| Annexin V的~(125)I标记及其在正常小鼠体内的分布 |
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| 引用本文： | 靳彩玲,许玉杰,范我,华子春,胡明江,王道锦,朱然,张友九.Annexin V的~(125)I标记及其在正常小鼠体内的分布[J].同位素,2005(4). |
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| 作者姓名： | 靳彩玲 许玉杰 范我 华子春 胡明江 王道锦 朱然 张友九 |
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| 作者单位： | 苏州大学放射医学与公共卫生学院 江苏苏州215007(靳彩玲，许玉杰，范我，胡明江，王道锦，朱然，张友九)，南京大学医药生物技术国家重点实验室 江苏南京210093(华子春) |
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| 基金项目： | 江苏省高校自然科学基金(04KJB350130)，江苏省核医学与生物技术重点实验室开放基金(KJS01010)资助项目 |
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| 摘 要： | 采用Iodogen法对Annexin V进行了125I标记,并观察了其在正常小鼠体内的分布情况。标记结果显示,125I-Annexin V标记率达94.9%,纯化后放化纯度达99%;室温放置72 h后,放化纯度仍保持在92%以上,表明其体外稳定性较好。生物分布结果显示,125I-Annexin V在肾脏中放射活性最高,其次为血液、肝脏、心、肺、脾;脑不吸收125I-Annexin V;肌肉、骨骼组织摄取亦较少;各组织、器官放射性摄取在1 h内除血液下降稍慢外,其余均有明显下降。表明125I-Annexin V适合用作核医学诊断试剂。
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| 关 键 词： | 125I标记 AnnexinV 生物分布 |
Labeling of Annexin V With ~(125)I and Its Biodistribution in Mice |
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| JIN Cai-ling,XU Yu-jie,FAN Wo,HUA Zi-chun,HU Ming-jiang,WANG Dao-jin,ZHU Ran,ZHANG You-jiu.Labeling of Annexin V With ~(125)I and Its Biodistribution in Mice[J].Isotopes,2005(4). |
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| Authors: | JIN Cai-ling XU Yu-jie FAN Wo HUA Zi-chun HU Ming-jiang WANG Dao-jin ZHU Ran ZHANG You-jiu |
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| Affiliation: | JIN Cai-ling~1,XU Yu-jie~1,FAN Wo~1,HUA Zi-chun~2,HU Ming-jiang~1,WANG Dao-jin~1,ZHU Ran~1,ZHANG You-jiu~1 |
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| Abstract: | Annexin V is labeled with ~(125)I by using Iodogen method and the biodistribution of which in mice is investigated.The labeling yield of ~(125)I-Annexin V is 94.9% and its radiochemical purity is over 99% after parification.The radioactivity uptake is the highest in kidney,followed in order by blood,liver,heart,lung and spleen;no uptake in brain;the lower in muscle and bone;The uptake of the selected tissues is decreased rapidly at 1 hour after injection except blood.The results show that it is a excellent imaging agent for diagnoses. |
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| Keywords: | ~(125)I labeling Annexin V biodistribution |
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