Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging |
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Authors: | Matthews Sinéad M Elder Alan D Yunus Kamran Kaminski Clemens F Brennan Colin M Fisher Adrian C |
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Affiliation: | Department of Chemical Engineering, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK. |
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Abstract: | A novel microfluidic approach for the quantification of reaction kinetics is presented. A three-dimensional finite difference numerical simulation was developed in order to extract quantitative kinetic information from fluorescence lifetime imaging experimental data. This approach was first utilized for the study of a fluorescence quenching reaction within a microchannel; the lifetime of a fluorophore was used to map the diffusion of a quencher across the microchannel. The approach was then applied to a more complex chemical reaction between a fluorescent amine and an acid chloride, via numerical simulation the bimolecular rate constant for this reaction was obtained. |
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