Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface |
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Authors: | Daugherty Patrick S; Olsen Mark J; Iverson Brent L; Georgiou George |
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Affiliation: | Departments of Chemical Engineering and
1 Chemistry and Biochemistry and
2 Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA |
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Abstract: | Polypeptide library screening technologies are critically dependentupon the characteristics of the expression system employed.A comparative analysis of the lpplac, tet and araBAD promoterswas performed to determine the importance of tight regulationand expression level in library screening applications. Thesurface display of single-chain antibody (scFv) in Escherichiacoli as an LppOmpA' fusion was monitored using a fluorescentlytagged antigen in conjunction with flow cytometry. In contrastto the lpplac promoter, both tet and araBAD promoterscould be tightly repressed. Tight regulation was found to beessential for preventing rapid depletion of library clones expressingfunctional scFv and thus for maintaining the initial librarydiversity. Induction with subsaturating inducer concentrationsyielded mixed populations of uninduced and fully induced cellsfor both the tet and araBAD expression systems. In contrast,homogeneous expression levels were obtained throughout the populationusing saturating inducer concentrations and could be adjustedby varying the induction time and plasmid copy number. Underoptimal induction conditions for the araBAD system, proteinexpression did not compromise either cell viability or librarydiversity. This expression system was used to screen a libraryof random scFv mutants specific for digoxigenin for clones exhibitingimproved hapten dissociation kinetics. Thus, an expression systemhas been developed which allows library diversity to be preservedand is generally applicable to the screening of E.coli surfacedisplayed libraries. |
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Keywords: | araBAD/ polypeptide library/ scFv/ FACS/ surface display/ tet |
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