| 从发酵液中提取达托霉素的工艺优化 |
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| 引用本文： | 常亮,魏增辉,李敏娜,梁冬,仲伟潭. 从发酵液中提取达托霉素的工艺优化[J]. 化学与生物工程, 2013, 30(7): 86-87 |
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| 作者姓名： | 常亮 魏增辉 李敏娜 梁冬 仲伟潭 |
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| 作者单位： | 微生物药物国家工程研究中心 河北省工业微生物代谢工程技术研究中心华北制药集团新药研究开发有限责任公司,河北 石家庄,050015 |
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| 摘 要： | 通过对迭托霉素发酵液预处理、溶剂萃取、结晶、干燥得到达托霉素粗品。确定了以pH值6．5～7．5的缓冲溶液作为浸提溶剂、加入5～8BV丙酮进行冷藏结晶的优化提取工艺。该工艺简单、经济，适合工业化生产。
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| 关 键 词： | 达托霉素 发酵液 萃取 结晶 |
Optimization of the Extraction Process of Daptomycin from Fermentation Broth |
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| CHANG Liang , WEI Zeng-hui , LI Min-na , LIANG Dong , ZHONG Wei-tan. Optimization of the Extraction Process of Daptomycin from Fermentation Broth[J]. Chemistry & Bioengineering, 2013, 30(7): 86-87 |
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| Authors: | CHANG Liang WEI Zeng-hui LI Min-na LIANG Dong ZHONG Wei-tan |
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| Affiliation: | (National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & Technology Research Center,New Drug Research & Development Company of NCPC,Shijiazhuang 050015,China) |
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| Abstract: | Daptomycin crude was obtained from fermentation broth through pretreatment,extraction, crys- tallization and dry. The optimal extraction process was determined as follows：the buffer solution with pH value of 6.5-7.5 was selected as extraction solvent, the cold crystallization was done with adding 5-8 BV of acetone. The process is simple, economic, suitable for industrial production. |
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| Keywords: | daptomycin fermentation broth extraction crystallization |
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