| 不同脂肪酶催化亚麻油水解反应性能的比较 |
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| 引用本文： | 王英雄,侯相林,王国富,杜俊民,乔欣刚,秦张峰,王建国. 不同脂肪酶催化亚麻油水解反应性能的比较[J]. 中国油脂, 2003, 28(8): 54-56 |
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| 作者姓名： | 王英雄 侯相林 王国富 杜俊民 乔欣刚 秦张峰 王建国 |
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| 作者单位： | 中国科学院山西煤炭化学研究所,030001,太原市165信箱 |
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| 基金项目： | 山西省青年科技研究基金(20011013) |
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| 摘 要： | 从反应温度、水／亚麻油摩尔比、脂肪酶用量等方面比较了L-lipase和N—1ipase脂肪酶在无溶剂体系中对亚麻油水解反应的催化性能。得到了两种脂肪酶的最佳使用温度及催化亚麻油水解反应的适宜条件。结果表明，两种脂肪酶的最佳用量均为3％～5％，适宜的水／亚麻油摩尔比为30：1。L-lipase和N-lipase的最佳温度分别是35℃和67．5℃，与L-lipase相比，N—1ipase的热稳定性和催化活性相对较高。
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| 关 键 词： | 脂肪酶 亚麻油 水解 催化性能 脂肪酸 |
| 文章编号： | 1003-7969(2003)08-0054-03 |
| 修稿时间： | 2003-02-26 |
Comparison of the Catalytic Property of Different Lipases in Linseed Oil Hydrolysis Reaction |
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| WANG Ying-xiong,HOU Xiang-lin,WANG Guo-fu,DU Jun-min,QIAO Xin-gang,QIN Zhang-feng,WANG Jian-guo. Comparison of the Catalytic Property of Different Lipases in Linseed Oil Hydrolysis Reaction[J]. China Oils and Fats, 2003, 28(8): 54-56 |
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| Authors: | WANG Ying-xiong HOU Xiang-lin WANG Guo-fu DU Jun-min QIAO Xin-gang QIN Zhang-feng WANG Jian-guo |
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| Abstract: | Catalytic abilities of lipase were studied in the solvent-free system, and hydrolysis of linseed oil was employed as a model reaction. The effects of the temperature, amount of enzyme and molar ratio of water and linseed on the conversions were examined. The optimal activity temperature of lipases and reaction condition for the hydrolysis of linseedoil were given. |
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| Keywords: | lipase linseed oil fatty acid hydrolysis solvent-free system |
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