| DL-苯甘氨酸的高效液相色谱分析 |
| |
| 引用本文： | 高宏,温锡敏,乔旭,崔咪芬,汤吉海.DL-苯甘氨酸的高效液相色谱分析[J].精细石油化工,2007,24(4):63-67. |
| |
| 作者姓名： | 高宏 温锡敏 乔旭 崔咪芬 汤吉海 |
| |
| 作者单位： | 南京工业大学化学化工学院,江苏,南京,210009;南京工业大学化学化工学院,江苏,南京,210009;南京工业大学化学化工学院,江苏,南京,210009;南京工业大学化学化工学院,江苏,南京,210009;南京工业大学化学化工学院,江苏,南京,210009 |
| |
| 摘 要： | 针对以苯甲醛、氯仿为原料合成苯甘氨酸的反应过程,建立了高效液相色谱法测定苯甘氨酸含量的方法,考察了流动相组成与流量对分离效果的影响,得出了适宜的分析条件。以10%甲醇+90%磷酸二氢钠溶液(20 mmol/L)为流动相,在其流量1 mL/min、柱温为室温和检测波长260 nm的条件下,苯甘氨酸质量浓度在25～400 mg/L范围内,质量浓度与峰面积呈较好的线性关系,系统精密度指标RSD值为0.13%。
|
| 关 键 词： | 分析 苯甘氨酸 高效液相色谱 |
| 收稿时间： | 2007-03-12 |
| 修稿时间： | 2007-03-122007-06-25 |
ANALYSIS OF DL-PHENYLGLECINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| |
| Gao Hong,Wen Ximin,Qiao Xu,Cui Mifen,Tang Jihai.ANALYSIS OF DL-PHENYLGLECINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Speciality Petrochemicals,2007,24(4):63-67. |
| |
| Authors: | Gao Hong Wen Ximin Qiao Xu Cui Mifen Tang Jihai |
| |
| Affiliation: | College of Chemistry and Chemical Engineering Nanjing University of Technology, Nanjing 210009, Jiangsu, China |
| |
| Abstract: | The high performance liquid chromatography(HPLC)method for the analysis of phenylg- lycine in the synthesis of phenylglycine from benzaldehyde and chloroform was established.The influ- ences of the composition and the flow-rate of the mobile phase on the separation were examined.The optimum chromatography conditions were found as follows.V(methanol):Vmonosodium phosphate (20 mmol/L)]=1:9 as the mobile phase,flow-rate of mobile phase 1 mL/min,detection wavelength 260 nm.The standard curve for phenylglycine was linear over the range of 25-400 mg/L.The RSD value for the accuracy of the system was 0.13%. |
| |
| Keywords: | analysis phenylglycine high performance liquid chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
