Combination of natural antimicrobials and sodium dodecyl sulfate for disruption of biofilms formed by contaminant bacteria isolated from sugarcane mills |
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| Affiliation: | 1. Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India;2. Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa 516003, India;1. Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China;2. Department of Urology, Affiliated Hospital of Nantong University, Nantong, 226001, China;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China;2. Jiangxi Province Key Laboratory of Edible and Medicinal Resources Exploitation, Nanchang University, Nanchang, 330031, China;3. School of Resource and Environmental and Chemical Engineering, Nanchang University, Nanchang, 330031, China;4. School of Food Science and Technology, Nanchang University, Nanchang, 330031, China;5. New Zealand Institute of Natural Medicine Research, 8 Ha Crescent, Auckland, 2104, New Zealand |
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| Abstract: | Bacterial contamination is found during ethanol production in the form of biofilms, which can decrease ethanol yield by 30%. Carvacrol, nisin, and hops extract, alone and in combination with SDS, were tested against planktonic and preformed biofilms of Lactobacillus fermentum, Lactobacillus plantarum, and/or Leuconostoc mesenteroides. MICs of carvacrol, nisin, hops extract and SDS were 250, 30, 5 and 37.5 mg/L, respectively, against all bacteria. Synergism between SDS and carvacrol (FICindex = 0.125), and SDS and nisin (FICindex = 0.25) was also found against all bacteria. Bacterial viability within biofilms and removal of biomass were indirectly measured using MTT and crystal violet methods, respectively. Metabolic activity of biofilms was inhibited up to 92% with nisin + SDS, while carvacrol + SDS could remove 82% of the biomass. Thus, combined use of these antimicrobials and SDS may provide an effective method for cleaning equipment of ethanol plants and prevent formation of biofilms. |
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