肾综合征出血热灭活疫苗的纯化

目的为提高肾综合征出血热（ＨＦＰＳ）疫苗的免疫效果和减少副反应。方法用ＥＬＩＳＡ检测ＨＦＲＳ 病毒抗原的方法，比较疫苗原液通过不同孔径滤膜的浓缩效果；比较离子交换和凝胶过滤的纯化效果。结果用 孔径３０万相对分子质量滤膜浓缩时，过滤液中仍有部分病毒抗原检出，经１０万相对分子质量滤膜过滤后，过滤液 中检测不到病毒抗原；用 Ｓｅｐｈａｒｏｓｅ ４ＦＦ凝胶柱层析浓缩时，经波长 ２８０ ｎｍ检测到 ３个吸收峰，其中仅在第 １峰中检 测到病毒抗原，收集第１峰，几乎回收全部病毒抗原，去除杂蛋白约９０％左右。结论本纯化方法适合于ＨＦＲＳ疫 苗的规模化生产。

Purification of Inactivated HFBS Vaccine

Objective To improve the immune effect and reduce the adverse reaction of inactivated HFRS vaccine.Methods The concentration effects of stock solution of vaccine by filter membrane with vari- ous sizes were compared by detecting HFRS virus antigen by ELISA. The purification effects of ion exchange chromatography and gel filtration were also compared. Results HFRS virus antigen could he detected out in the stock solution concentrated by the filter membrane with a size of Mr 300 000, but could not be detected out in that concentrated by the filter membrane with a size of Mr 100 000. After the stock solution were purified by Sepharose 4FF gel column chromatography , 3 peaks were observed on the profile at a wavelength of 280nm. However, virus antigen was only detected out in the first peak. Almost all the virus antigens were recovered in the peak, and about 90% of heterologous proteins were removed. Conclusion The purification method is sim- ple, economic and suitable for the large scale production of purified HFRS vaccine.