不同产地裸燕麦代谢产物差异分析

目的：探究不同地区同一品种裸燕麦中的代谢产物及其差异。方法：以代谢组学研究方法为基础，利用甲醇提取极性代谢组分，三氟乙酰胺(BSTFA)进行衍生化处理，并通过GC-MS技术和NIST数据库确定代谢产物。结果：在两个产地同一品种的裸燕麦中共检测出56种代谢产物，HZ的代谢产物占38种，HQ的代谢产物占45种，包括各种碳水化合物和少量次生代谢物。结论：两个地区的燕麦代谢产物及其含量基本一致，均属于常见化合物。但其中亚油酸、反油酸和棕榈酸等常见化合物的浓度不同，D-葡萄糖酸和吡喃半乳糖等代谢产物具有特异性。

Analysis on the difference of metabolites of naked oats from different produced areas

Objective:Zhangjiakou naked oats in Hebei province (HZ) and Qiqihar naked oats in Heilongjiang province (HQ) were selected as the research objects. The metabolites and differential metabolites of naked oats of the same variety in different regions were explored.Methods:Metabolomics research methods were used as the basis. Methanol was used to extract polar metabolic components, with Bis(trimethylsilyl)trifluoroacetamide (BSTFA) selected for derivatization, and the metabolites were determined through GC-MS technology and the NIST database.Results:A total of 56 kinds of metabolites were detected, including various carbohydrates and a small amount of secondary metabolites, in which HZ accounted for 38 kinds and HQ accounted for 45 kinds.Conclusion:The oat metabolites and their contents in the two regions were basically the same, which were common compounds. However, the concentrations of common compounds such as linoleic acid, elaidic acid, and palmitic acid were different, and metabolites such as D-gluconic acid and galactopyranose were specific.