商品化酶抑制-比色法农残速测试剂盒对农药的敏感性测定

探讨商品化的酶抑制-比色法农残速测试剂盒对50种农药的敏感性测定.使用商品化的酶抑制.比色法试剂盒对嘧啶磷等40种有机磷和10种氨基甲酸酯类农药残留进行5个浓度水平的测定;对有3个浓度水平的抑制率大于0的农药,用抑制率和农药浓度的常用对数进行线性拟合,得出相关系数和抑制率方程,并据此计算IC10和IC50,通过IC10...

Determination of Pesticide Sensitivity on Commercial Enzyme Inhibition Colorimetric Pesticide Residue Test Kit

To investigate the sensitivity of commercial enzyme inhibitor-colorimetry agrochemical residue test kit to 50 kinds of pesticides.It was tested with commercial enzyme inhibition colorimetric kit at five concentration levels in 40 kinds of organophosphorus and 10 kinds of carbamate pesticide residues,such as pyrimidine phosphate.For pesticides with inhibition rates greater than 0 at three concentration levels,the common logarithm of inhibition rate and pesticide concentration was used for linear fitting to obtain the correlation coefficient and inhibition rate equation,and then IC10 and IC50 were calculated accordingly,and the sensitivity of the kit to various pesticides was compared by IC10.The selected kits have different sensitivities to each pesticide.For IC10,there is also a difference between 3 mL and 2.5 mL sample solutions,and the difference of individual pesticides is one or two orders of magnitude.It is recommended that users test the kit as needed when purchasing the kit.