| 一种用于枯草杆菌发酵生产四甲基吡嗪的补料策略 |
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| 引用本文： | 朱兵峰,徐岩.一种用于枯草杆菌发酵生产四甲基吡嗪的补料策略[J].酿酒科技,2011(2):17-22. |
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| 作者姓名： | 朱兵峰 徐岩 |
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| 作者单位： | 1. 江南大学食品科学与技术国家重点实验室,工业生物技术教育部重点实验室,生物工程学院,江苏,无锡,214122;山东新时代药业有限公司,山东,费县,273400
2. 江南大学食品科学与技术国家重点实验室,工业生物技术教育部重点实验室,生物工程学院,江苏,无锡,214122 |
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| 基金项目： | 国家支撑计划(2007BAK36B00、2008BAI63B06); 长江学者和创新团队发展计划(IRT0532); 111工程(111-2-6); 国家自然科学基金(NS-FCNo.30470046,20872050); 江南大学食品科学与技术国家重点实验室的资助 |
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| 摘 要： | 考察了铵盐对Bacillus subtilis CCTCC M 208157发酵产TTMP的影响,发现磷酸铵盐(DAP)对发酵体系中TTMP的合成有显著促进作用。进一步考察了TTMP合成对DAP的需求,结果表明,高浓度的DAP有利于TTMP的合成,但高浓度的铵根离子对细胞的生长和前体(3-羟基丁酮,乙偶姻)有抑制作用。基于以上结果,建立了一种铵盐A策略,并应用于摇瓶发酵和7.5-l发酵罐培养,TTMP浓度达7.46 g/L和7.34 g/L,与分批发酵相比分别提高了55.1%和29.0%。
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| 关 键 词： | 微生物 TTMP 3-羟基-2-丁酮 铵盐 枯草杆菌 |
A Feeding Strategy for Tetramethylpyrazine Production by Bacillus subtilis based on the Stimulating Effects of Ammonium Phosphate |
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| ZHU Bing-feng,XU Yan.A Feeding Strategy for Tetramethylpyrazine Production by Bacillus subtilis based on the Stimulating Effects of Ammonium Phosphate[J].Liquor-making Science & Technology,2011(2):17-22. |
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| Authors: | ZHU Bing-feng XU Yan |
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| Affiliation: | ZHU Bing-feng1,2 and XU Yan1 (1.State Key Lab of Food Science and Technology,Key Lab of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122,2.Shangdong New Times Pharmaceutical Co.Ltd.,Feixian,Shandong 273400,China) |
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| Abstract: | In order to investigate the effects of ammonium salts on tetramethylpyrazine(TTMP) production by Bacillus subtilis CCTCC M 208157,different ammonium salts concentration was tested,and diammonium phosphate(DAP) was found to have a predominant effect on stimulating TTMP synthesis.Then DAP requirements for TTMP production was further investigated,the experimental results showed that higher concentration of DAP favored TTMP production,while both ammonium and phosphate ions exhibited inhibitory effects on the ce... |
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| Keywords: | microbe Tetramethylpyrazine 3-hydroxy-2-butanone ammonium salt Bacillus subtilis |
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