高压液相色谱-质谱法和高效液相色谱法分析籽瓜中环磷酸腺苷

采用高压液相色谱-质谱法鉴定籽瓜中的环磷酸腺苷，并建立高效液相色谱法测定籽瓜中环磷酸腺苷的含量，对样品提取条件进行优化。结果表明：环磷酸腺苷在0.1～100 mg/L质量浓度范围内线性关系良好，相关系数为0.999 9；平均加标回收率为98.40%，相对标准偏差为3.22%。经单因素试验和正交试验，确定优化的提取条件为料液比1∶12（鲜样）和1∶60（干样）水提取，提取温度90 ℃、提取时间40 min、提取次数3 次。样品前处理和分析方法准确、简便、可靠、重复性好，可用于籽瓜中环磷酸腺苷含量的分析测定。

Identification and Quantitation of Cyclic Adenosine 3’,5’-monophosphate in Seeding Watermelon by Liquid Chromatography-Tandem Mass Spectrometry and High Performance Liquid Chromatography

A method to identify cAMP using liquid chromatography coupled to tandem mass spectrometry (LC-MSMS)
and to quantify the content of cAMP in seeding watermelon using high performance liquid chromatography (HPLC)
was established. The quantitative method showed a good linear relationship in the cAMP concentration range from 0.1 to
100 mg/L with a correlation coefficient of 0.999 9. The average recovery for spiked samples was 98.40% with relative
standard deviation (RSD) of 3.22% (n = 5). By using one-factor-at-a-time and orthogonal array designs, the optimum
conditions for cAMP extraction were determined as follows: solid-to-water ratio; 1:12 (fresh sample) or 1:60 (dry sample);
temperature, 90 ℃; extraction time, 40 min. This method is simple, accurate, reliable and reproducible, and can be used for
the determination of cAMP in seeding watermelon.