短瓣金莲花不同提取部位的抗氧化活性

目的：研究短瓣金莲花的抗氧化活性部位。方法：将短瓣金莲花药材依次用石油醚、乙酸乙酯、95%乙醇、60%乙醇、30%乙醇和去离子水提取，采用紫外-可见分光光度法对不同溶剂提取部位的黄色素、总黄酮和总酚含量进行分析，并测试各提取部位对羟自由基（•OH）和1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基的清除作用以对其抗氧化活性进行研究。结果：短瓣金莲花石油醚提取部位的黄色素含量最高，为（11.37±0.07 ）mg/g；而60%乙醇提取部位总黄酮和总酚含量最高，分别为（213.21±1.12）mg/g和（121.75±0.58） mg/g，95%乙醇提取部位次之，分别为（200.47±0.65）mg/g和（105.19±0.61）mg/g。石油醚提取部位清除•OH能力最强，半抑制浓度（50% inhibiting concentration，IC50）为92.77 mg/L；95%乙醇提取部位清除DPPH自由基能力最强，IC50为10.14 mg/L，60%乙醇提取部位次之，IC50为10.70 mg/L。结论：短瓣金莲花清除•OH能力与黄色素含量呈正相关性，而清除DPPH自由基能力与黄酮和多酚含量呈正相关性；短瓣金莲花的石油醚和95%乙醇提取部位是最佳的抗氧化活性部位，有待进一步深入研究。

Antioxidant Activity of Different Solvents Extracts from Flowers of Trollius ledebouri Reichb

Purpose: To explore the antioxidant activity of different solvent extracts from the flowers of Trollius ledebouri
Reichb. Methods: The dried flowers were extracted sequentially with petroleum ether, ethyl acetate, 95% ethanol, 60%
ethanol, 30% ethanol and deionized water. Each extract obtained was analyzed by ultraviolet-visible spectrophotometry for
the contents of yellow pigment, total flavonoids and total polyphenols, and their radical scavenging activity against hydroxyl
and DPPH free radicals were tested. Results: Petroleum ether extract had the highest content of yellow pigment (11.37 ±
0.07) mg/g; 60% ethanol extract from the flowers of Trollius ledebouri had the highest content of total flavonoids (213.21 ±
1.12) mg/g and total polyphenols (121.75 ± 0.58) mg/g, 95% ethanol extract had slightly lower contents of total flavonoids
(200.47 ± 6.49) mg/g and total polyphenols (105.19 ± 0.61) mg/g. Petroleum ether extract had the strongest hydroxyl
radical scavenging ability with IC50 of 92.77 mg/L, while 95% ethanol extract had the strongest DPPH radical scavenging
ability with IC50 of 10.14 mg/L and 60% ethanol extract had slightly lower DPPH radical scavenging ability with IC50 of
10.70 mg/L. Conclusion: The hydroxyl radical scavenging capacity presented a positive correlation with the concentration
of yellow pigment, and the DPPH radical scavenging capacity presented a positive correlation with the concentration of
total flavonoids and total polyphenols; petroleum ether and 95% ethanol extracts from the flowers of Trollius ledebouri have
potent antioxidant activity.