啤酒花总黄酮测定方法的比较研究

以高效液相色谱法对啤酒花总黄酮含量的测定结果作为评判标准（相对准确值），比较与评价AlCl3分光光度法、Al(NO3)3分光光度法和硼氢化钠/氯醌分光光度法3 种比色法测定酒花总黄酮含量的准确性。结果表明，高效液相色谱法测定酒花总黄酮含量为（12.73±0.26） mg/g；AlCl3法测得总黄酮含量为（13.66±0.45）mg/g，与高效液相色谱法偏差率为7.3%；而Al(NO3)3法测定结果为（43.53±1.16）mg/g，与高效液相色谱法的偏差率为241.9%；硼氢化钠/氯醌法测定结果为（70.25±1.42）mg/g，与高效液相色谱法的偏差率为451.8%。提出AlCl3分光光度法比其他2 种比色法更适用于酒花总黄酮含量的测定，该方法误差小，准确性好。

Comparative Study on Assays for Determination of Flavonoids in Hops (Humulus lupulus L.)

The accuracy of three spectrophotometric assays for the determination of flavonoids in hops
(Humulus lupulus L.) using respectively AlCl3, Al(NO3)3 and sodium borohydride/chloranil (SBC) was evaluated by
comparison with the reference data (relatively more accurate) from HPLC analysis. The results showed that the total content
of flavonoids in hops was (12.73 ± 0.26) mg/g by HPLC, compared to (13.66 ± 0.45) mg/g, (43.53 ± 1.16) mg/g, and
(70.25 ± 1.42) mg/g with a relative deviation of 7.3%, 241.9% and 451.8%, respectively, as spectrophotometrically assayed
using AlCl3, Al(NO3)3 and SBC. Therefore, the AlCl3-based assay is proposed to be more suitable for the determination of
flavonoids in hops with smaller errors.