基于高效薄层色谱法的胡麻卵磷脂质量分析

目的：建立胡麻卵磷脂的高效薄层色谱质量分析方法。方法：将卵磷脂供试品及磷脂酰胆碱标准品溶于氯仿-甲醇（3∶2，V/V），以氯仿-甲醇-水（65∶25∶4，V/V）为展开系统，考察不同显色剂、展开系统、点样量、薄层板、检视方式、温度、相对湿度对卵磷脂供试品中不同组分分离的影响，进行高效薄层色谱定性分析；在确定的色谱条件下，将卵磷脂供试品及磷脂酰胆碱标准品进行展开，扫描磷脂酰胆碱峰面积，绘制标准曲线，外标法测定卵磷脂供试品中磷脂酰胆碱的含量。结果：卵磷脂供试品各组分分离情况良好，适于定性分析；卵磷脂供试品中磷脂酰胆碱的含量为63.33%，板间、板内精密度实验相对标准偏差（relative standard deviation，RSD）分别为1.82%和3.73%，稳定性实验RSD为1.60%，加标平均回收率为98.5%。结论：建立的方法快速准确、重复性好，可为卵磷脂质量分析提供新的技术参考。

Quality Analysis of Flax Lecithin Based on High Performance Thin Layer Chromatography

Objective: To establish a method for the quality analysis of lecithin extracted from flax by high performance
thin layer chromatography (HPTLC). Methods: Test samples of lecithin and phosphatidylcholine standard were dissolved
separately in chloroform-methanol (3:2, V/V) before analysis by HPTLC. The developing system consisted of chloroform,
methanol and water (65:25:4, V/V). The effects of chromogenic agents, developing systems, amounts of spotted sample,
thin layer chromatography plates, temperature and relative humidity on the separation of each component in sample were
investigated. Under the best chromatography condition, the lecithin in the sample and the standard were developed, and
the peak area of phosphatidylcholine was scanned. A standard curve was established and phosphatidylcholine in sample
was quantified by an external standard method. Results: The separation of each component in sample was suitable for
qualitative analysis. The average content of phosphatidylcholine in lecithin sample was 63.33% with plate-to-plate and
within-plate precision (relative standard deviation, RSD) of 1.82% and 3.73%, respectively, and stability (RSD) of 1.60%,
and the average recovery was 98.5%. Conclusion: The method is rapid, accurate and reproducible, which can provide a new
technical reference for the quality analysis of lecithin.