利用核磁共振法定量分析植物油中多种脂肪酸及水含量

采用核磁共振波谱分析技术，归属了植物油中亚麻酸、亚油酸、油酸等不饱和脂肪酸甘油酯、饱和脂肪酸甘油酯及水的核磁共振质子谱的主要特征峰。在无需预处理条件下，以苯甲酸作为内标，对植物油中各种脂肪酸及水进行定量分析，加标回收率可达98%～102%。亚麻酸、亚油酸、油酸、总饱和脂肪酸、水的精密度分别为0.11%、0.12%、0.68%、0.70%、2.65%。该法取样量少、无需昂贵的标样，可直接快速定量，结果准确，可为植物油的质量标准研究提供一种简单可行的新方法。

Quantitative Analysis of Fatty Acids and Water in Vegetable Oils by Nuclear Magnetic Resonance

The main characteristic peaks of linolenic, linoleic, oleic acid, saturated fatty acids and water in vegetable oils
were analyzed by 1H-NMR spectroscopy. Benzoic acid was selected as an internal standard to quantify the contents of
linolenic, linoleic, oleic acid, saturated fatty acids and water in vegetable oils by this method without any pretreatment. The
recoveries were 98%–102% with RSD of 0.11%, 0.12%, 0.68%, 0.70% and 2.65% for linolenic, linoleic, oleic acid, total
saturated fatty acids and water, respectively. The contents of linolenic, linoleic, oleic acid, total saturated fatty acids and
water can be determined directly and accurately with less sample consumption and without using expensive standards. This
new method is simple and applicable for studying quality standards for vegetable oils.