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| 马奶及其制品中肠球菌属乳酸菌的安全性评价 | |
| 引用本文： | 王梦姣,李少英,李淑芬,贺文英,宋晓敏,马春艳,李贞,骆袁. 马奶及其制品中肠球菌属乳酸菌的安全性评价[J]. 食品科学, 2014, 35(17): 204-208. DOI: 10.7506/spkx1002-6630-201417039 |
| 作者姓名： | 王梦姣 李少英 李淑芬 贺文英 宋晓敏 马春艳 李贞 骆袁 |
| 作者单位： | 1.内蒙古农业大学食品科学与工程学院，内蒙古 呼和浩特 010018；2.内蒙古农业大学生命科学学院，内蒙古 呼和浩特 010018；3.内蒙古农业大学理学院，内蒙古 呼和浩特 010018；4.呼和浩特铁路局，内蒙古 呼和浩特 010057 |
| 基金项目： | 国家自然科学基金地区科学基金项目(31060014) |
| 摘 要： | 目的：为使具有民族特色和特殊功效的马奶及其制品中的有益乳酸菌应用于食品发酵工业，确保分离自内蒙古牧区靠自然发酵制作的马奶及其制品中的乳酸菌的安全性，对分离自马奶及其制品中的乳酸菌进行安全性评价。方法：通过吲哚实验、硝酸盐还原酶实验、氨基脱羧酶实验、溶血实验、D-乳酸检测及质粒提取实验，确定菌株是否含有有害代谢产物及有无耐药性。结果：菌株的吲哚实验、硝酸盐还原酶实验、氨基脱羧酶实验、溶血实验均为阴性，在菌株JHZ9、JHZ15、JHZ25、JNN1中检出了少量的D-乳酸，菌株对实验所选药品无耐药性且未检测到可转移的耐药质粒。结论：7 株肠球菌属的乳酸菌在代谢活动中不产生有害代谢产物，不存在可转移的耐药质粒。 |
| 关 键 词： | 乳酸菌 安全性 有害代谢产物 耐药性 质粒 |
Safety Assessment of Enterococcus Isolated from Mare’s Milk and Its Products |
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| WANG Meng-jiao,LI Shao-ying,LI Shu-fen,HE Wen-ying,SONG Xiao-min,MA Chun-yan,LI Zhen,LUO Yuan. Safety Assessment of Enterococcus Isolated from Mare’s Milk and Its Products[J]. Food Science, 2014, 35(17): 204-208. DOI: 10.7506/spkx1002-6630-201417039 | |
| Authors: | WANG Meng-jiao LI Shao-ying LI Shu-fen HE Wen-ying SONG Xiao-min MA Chun-yan LI Zhen LUO Yuan |
| Affiliation: | 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. College of LifeSciences, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. College of Science, Inner Mongolia AgriculturalUniversity, Hohhot 010018, China; 4. Huhhot Railway Administration, Hohhot 010057, China |
| Abstract: | Objective: This study aimed to isolate lactic acid bacteria (LAB) from mare’s milk and its products, which have nationality characteristics and special functions. The mare’s milk and its products were made by natural fermentation in pastoral areas of Inner Mongolia. In order to ensure their safe application in the food fermentation industry, it is necessary to assess the safety of LAB isolated from mare’s milk and koumiss. Methods: Indole test, nitrate reductase assay, amino decarboxylase test, hemolysis test, D-lactic acid assay and plasmid extraction were performed to identify whether harmful metabolites or drug resistance exists in the tested strains. Results: Negative results were observed in all tests except for D-lactic acid, which was detected in small amounts in strains JHZ9, JHZ15, JHZ25 and JNN1. Neither resistance to the tested drugs nor transferable resistant plasmid was detected in seven tested strains. Conclusion: Neither harmful metabolites nor drug resistant plasmids has been detected in the seven strains, which belong to Enterococcus. |
| Keywords: | lactic acid bacteria safety harmful metabolites drug resistance plasmids |
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