QuEChERS-高效液相色谱-质谱法检测食品中14 种真菌毒素

建立QuEChERS-高效液相色谱-质谱检测食品中14 种真菌毒素的方法。均质样品用1%乙酸-乙腈提取，经分散固相萃取净化后，采用ACQUITU UPLC BEH C18色谱柱（2.1 mm×50 mm，1.7 μm）分离。采用电喷雾电离、多反应监测方式，可同时对食品中脱氧雪腐镰刀菌烯醇、青霉酸、黄曲霉毒素M1、黄曲霉毒素G2、桔青霉毒素、黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1、玉米赤霉烯酮、赭曲霉毒素A、杂色曲霉毒素、HT-2毒素、T-2毒素、鬼臼毒素14 种真菌毒素进行定性和定量分析。最低检出限为0.5～1 μg/kg。该方法简便快速、选择性佳、灵敏度高，适用于食品安全事件分析中真菌毒素的分析。

Simultaneous Screening for 14 Mycotoxin Contaminants in Foods by QuEChERS-LC-MS-MS

This paper presents a QuEChERS-liquid chromatography-tandem mass chromatography (LC-MS-MS) method
for the simultaneous screening for 14 mycotoxin contaminants (deoxynivalenol, penicillic acid, aflatoxin M1, aflatoxin G2,
citrinin, aflatoxin B1, aflatoxin B2, aflatoxin G1, zearalenone, ochratoxin A, sterigmatocystin, HT-2 toxin, T-2 toxin and
podophyllotoxin) in foods. The homogenized samples were extracted with 1% acetic acid in acetonitrile. The separation
was performed on an ACQUITU UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) by gradient elution after purification
by dispersive-solid-phase extraction (d-SPE). The 14 mycotoxin contaminants in foods analyzed by LC-MS-MS-ESI in
the positive ionization, multiple-reaction monitoring (MRM) mode. The minimum detection limit was 0.5–1 μg/kg. The
developed method proved to be simple, rapid, highly selective and sensitive, and suitable for the analysis of mycotoxin
contaminants in food safety incidents.