酶法提取蛋黄油的工艺

利用酶法从鸡蛋黄粉中提取蛋黄油 ,通过正交试验选出酶解法提取蛋黄油的最佳工艺条件 :底物质量浓度为 2 0 g/dL ,先用每 10 0g底物中加入 1g蛋白酶A ,并保持其最适 pH和温度 ,反应时间为 2h ;再用每 10 0 g底物中加入 1.5 g的蛋白酶B ,并同时调节到相应的最适 pH和温度 ,反应时间为 2h ,该条件下蛋黄油得率为 5 8.89% .最后 ,对蛋黄油的主要成分进行了分析

Enzymatic Extraction of Egg Yolk Oil

In this article, the procedure of extracting egg yolk oil from egg yolk powder by enzymes was studied. Through orthogonal experiments, the best technical conditions of enzymatic extraction of egg yolk oil were determined as followings:the concentration of substrate was 20%. At the optimum pH and temperature of proteinase A,1% of proteinase A (Wt%, proteinase A vs substrate) was added and reacted for 2 h. Then at the optimum pH and temperature of proteinase B,1.5% of proteinase B(Wt%, proteinase B vs substrate) was added and reacted for 2 h. Under these conditions,the yield of egg yolk oil was 58.89%. In addition, the ingredients of egg yolk oil were also analyzed.