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红光对安卡红曲霉GZU4577的调控及其与无机氮源的相关性
引用本文:赵永霞,周礼红,谢健,王博语.红光对安卡红曲霉GZU4577的调控及其与无机氮源的相关性[J].中国酿造,2020,39(3):73.
作者姓名:赵永霞  周礼红  谢健  王博语
作者单位:(1.茅台学院 酿酒工程系,贵州 仁怀 564507;2.贵州大学 生命科学学院,贵州 贵阳 550025; 3.遵义医学院 遗传教研室,贵州 遵义 563000)
基金项目:贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]448号);仁怀市科技计划项目(仁怀支撑[2019]3号);大学生创新创业训练计划(遵医科院20173810)
摘    要:该研究基于无氮源察氏培养基,通过菌落观察、生长测量、分生孢子和闭囊壳计数及色价测定研究红光对安卡红曲霉(Monascus anka)GZU4577的调控及其与无机氮源的相关性。结果表明,在无氮源添加时,红光对菌株GZU4577的径向生长和各色素的色价无显著影响(P>0.05),但显著促进了分生孢子和闭囊壳的生成(P<0.05);添加无机氮源后,红光对菌株GZU4577的影响机制发生改变:添加硝酸钠(NaNO3)后,红光显著抑制菌株GZU4577的径向生长和分生孢子、闭囊壳的生成(P<0.05),但对各色素色价无显著影响(P>0.05)。添加氯化铵(NH4Cl)后,红光只抑制菌株GZU4577的闭囊壳和各色素的生成(P<0.05)。添加硝酸铵(NH4NO3)后,红光显著抑制了菌株GZU4577的闭囊壳和分生孢子、红色素和黄色素的生成(P<0.05)。因此,红光对菌株GZU4577的调控与氮源存在与否及氮源种类相关。

关 键 词:安卡红曲霉GZU4577  红光  氮源  调控  

Regulation of red light on Monascus anka GZU4577 and its relationship with nitrogen sources
ZHAO Yongxia,ZHOU Lihong,XIE Jian,WANG Boyu.Regulation of red light on Monascus anka GZU4577 and its relationship with nitrogen sources[J].China Brewing,2020,39(3):73.
Authors:ZHAO Yongxia  ZHOU Lihong  XIE Jian  WANG Boyu
Affiliation:(1.Department of Liquor-making Engineering, Moutai Institute, Renhuai 564507, China; 2.College of Life Sciences, Guizhou University, Guiyang 550025, China; 3.Department of Medical Genetics, Zunyi Medical University, Zunyi 563000, China)
Abstract:Based on Czapek medium without nitrogen source, the regulation of red light on Monascus anka GZU4577 and its relationship with nitrogen sources were studied by colony morphology observation, growth detection, conidia and cleistothecia counting, and color value detection. The results showed that the red light had no significant effect on the radial growth of strain GZU4577 and the color value of each pigments (P>0.05) without nitrogen source addition, but it significantly promoted the generation of conidia and cleistothecia (P<0.05). After adding inorganic nitrogen source, the effect mechanism of red light on strain GZU4577 was changed. After adding NaNO3, the red light significantly inhibited the radial growth of strain GZU4577 and the generation of conidia and cleistothecia (P<0.05), but had no significant effect on the color value of each pigments (P>0.05). After adding NH4Cl, the red light only inhibited the generation of cleistothecia and each pigments of strain GZU4577 (P<0.05). After adding NH4NO3, The red light significant inhibited the generation of cleistothecia, conidia, red pigment and yellow pigment of strain GZU4577 (P<0.05). Therefore, the regulation of red light on M. anka GZU4577 was related to the presence and types of nitrogen sources in the medium.
Keywords:Monascus anka GZU4577  red light  nitrogen source  regulation  
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