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茶多酚辅助植物乳杆菌FQR降解亚硝酸盐效应的研究
引用本文:潘冬梅,吴怡瑾,王玉露,李海燕,梅林. 茶多酚辅助植物乳杆菌FQR降解亚硝酸盐效应的研究[J]. 中国酿造, 2020, 39(5): 131. DOI: 10.11882/j.issn.0254-5071.2020.05.025
作者姓名:潘冬梅  吴怡瑾  王玉露  李海燕  梅林
作者单位:(安徽农业大学 茶与食品科技学院,安徽 合肥 230036)
基金项目:安徽省现代产业技术体系(生猪)建设项目支助(11008751);安徽省科技公关面上项目(17030701015);安徽省高等学校大学生创新创业项目(201610364010)
摘    要:该研究在含有NaNO2的植物乳杆菌FQR培养液中加入茶多酚(TPs),考察培养12 h后体系中亚硝酸盐降解率,以评价茶多酚辅助植物乳杆菌(Lactobacillus plantarum)降解亚硝酸盐的效果。结果表明,茶多酚对植物乳杆菌FQR的生长状况有一定的促进作用,添加量为0.2%时生长状况最佳,12 h时菌落数达到3.3×108 CFU/mL。进一步研究了不同培养条件对茶多酚辅助植物乳杆菌降解亚硝酸盐效应的影响,得出茶多酚添加量为0.3%时亚硝酸盐降解效果最好,为(83.05±1.48)%;接种量为3%时,亚硝酸盐降解率最高,为(89.09±0.63)%;培养温度为37 ℃时,亚硝酸盐降解率最高,为(88.23±0.59)%;起始pH值为6.0时,亚硝酸盐降解率最高,为(89.79±0.57)%。

关 键 词:植物乳杆菌  茶多酚  亚硝酸盐  降解  培养条件  

Effect of tea polyphenols-assisted Lactobacillus plantarum FQR on nitrite degradation
PAN Dongmei,WU Yijin,WANG Yulu,LI Haiyan,MEI Lin. Effect of tea polyphenols-assisted Lactobacillus plantarum FQR on nitrite degradation[J]. China Brewing, 2020, 39(5): 131. DOI: 10.11882/j.issn.0254-5071.2020.05.025
Authors:PAN Dongmei  WU Yijin  WANG Yulu  LI Haiyan  MEI Lin
Affiliation:(School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China)
Abstract:Tea polyphenols (TPs) were added to the Lactobacillus plantarum FQR culture solution contained NaNO2, the degradation rate of nitrite in the culture solution after 12 h was investigated to evaluate the effect of tea polyphenols on the degradation of nitrite by L. plantarum. The results showed that tea polyphenols promoted the growth of L. plantarum FQR, optimally when the addition was 0.2%, with colonies 3.3×108 CFU/ml at 12 h. Furthermore, the effects of different culture conditions on the degradation of nitrite by tea polyphenols-assisted L. plantarum FQR were explored. The optimal culture conditions were tea polyphenols addition 0.3%, inoculum 3%, culture temperature 37 ℃ and initial pH 6.0, the highest nitrite degradation rates were (83.05±1.48)%, (89.09±0.63)%, (88.23±0.59)%, and (89.79±0.57)%, respectively.
Keywords:Lactobacillus plantarum  tea polyphenols  nitrite  degradation  culture conditions  
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