盐渍藠头盐水细菌多样性及基因功能预测分析

采用MiSeq高通量测序技术对来凤地区盐渍藠头盐水细菌多样性进行解析，并采用PICRUSt技术对其基因功能进行预测。结果表明，99.58%的基因序列长度为425～451 bp，基本覆盖16S rRNA V3-V4区序列；优势细菌门为硬壁菌门（Firmicutes）、变形菌门（Proteobacteria）和放线菌门（Actinobacteria），平均相对含量分别为43.94%、33.12%和18.21%；优势细菌属为乳杆菌属（Lactobacillus）、假单胞菌属（Pseudomonas）和嗜热油菌属（Thermoleophilum），平均相对含量分别为30.28%、18.21%和16.13%；共有219个核心操作分类单元（OTU），包含的序列数占总序列数的75.56%；盐渍藠头盐水中可能共有大量具有较强氨基酸转运和代谢功能的细菌类群。

Bacterial diversity and gene functional prediction of saline Allium chinense G. Don brine

The bacterial diversity of saline Allium chinese G. Don brine samples collected from Laifeng area was studied by MiSeq high-throughput sequencing, and their gene functions were predicted by PICRUSt technique. The results indicated the lengths of 99.58% sequences were 425-451 bp, which basically cover the V3-V4 region sequence of 16S rRNA. Firmicutes, Proteobacteria and Actinobacteria were dominant bacterial phyla with the average abundances of 43.94%, 33.12% and 18.21%, respectively. Lactobacillus, Pseudomonas and Thermoleophilum were dominant bacterial genera with the average abundances of 30.28%, 18.21% and 16.13%, respectively. The sequences were contained by 219 OTUs, which shared by all samples, accounted for 75.56% of all sequences. The saline A. chinense G. Don brine samples shared a large number of core bacterial microbiome with potential functions of amino acid transport and metabolism.