QuEChERS-HPLC-MS/MS法测定保健药酒中的28种那非类物质的含量

建立了保健药酒中28种那非类物质的QuEChERS-HPLC-MS/MS检测方法，样品使用甲醇溶解，经N-丙基乙二胺净化后，经色谱柱分离，在电喷雾离子化正离子模式下，以多反应监测（MRM）模式检测，以保留时间和特征离子对定性，外标法定量。结果表明，28种目标物在10～200 μg/L范围内均呈良好的线性关系，相关系数（R2）≥0.992 0，加标回收率为90.3%～112.1%，相对标准偏差（RSD）为1.8%～5.3%，检出限为0.01～0.12 mg/L，定量限为0.03～0.36 mg/L。该方法定性定量准确，操作简便，适用于保健药酒中那非类物质的检测与确证。

Determination of 28 nafils substances content in health medicinal liquor by QuEChERS-HPLC-MS/MS

A QuEChERS-HPLC-MS/MS method for simultaneous determination of 28 nafils substances in health medicinal liquor was developed.The samples were dissolved in methanol,purified by N-propylethylenediamine,separated by chromatographic column,detected using electrospray ionization in the positive ion mode with multi-reaction monitoring(MRM)mode,quantified by retention time and characteristic ion pairs,and quantified by external standards.The results showed that the 28 targets had a good linear relationship in the range of 10-200μg/L,and the correlation coefficient(R2)≥0.9920.The adding standard recovery rate was 90.3%-112.1%,the relative standard deviation(RSD)was 1.8%-5.3%,the limits of detection was 0.01-0.12 mg/L,and the lowest quantitation limit was 0.03-0.36 mg/L.The established method was accurate and simple,which was suitable for the detection and confirmation of nafils substances in health medicinal liquor.